Lee C K, Klopp R G, Weindruch R, Prolla T A
Environmental Toxicology Center, Institute on Aging, Department of Genetics, University of Wisconsin, Madison, WI 53706, USA.
Science. 1999 Aug 27;285(5432):1390-3. doi: 10.1126/science.285.5432.1390.
The gene expression profile of the aging process was analyzed in skeletal muscle of mice. Use of high-density oligonucleotide arrays representing 6347 genes revealed that aging resulted in a differential gene expression pattern indicative of a marked stress response and lower expression of metabolic and biosynthetic genes. Most alterations were either completely or partially prevented by caloric restriction, the only intervention known to retard aging in mammals. Transcriptional patterns of calorie-restricted animals suggest that caloric restriction retards the aging process by causing a metabolic shift toward increased protein turnover and decreased macromolecular damage.
对小鼠骨骼肌衰老过程中的基因表达谱进行了分析。使用代表6347个基因的高密度寡核苷酸阵列显示，衰老导致了一种差异基因表达模式，表明存在明显的应激反应以及代谢和生物合成基因的表达降低。热量限制是已知唯一能延缓哺乳动物衰老的干预措施，大多数变化要么完全要么部分被热量限制所阻止。热量限制动物的转录模式表明，热量限制通过导致代谢向增加蛋白质周转和减少大分子损伤的方向转变来延缓衰老过程。
