心脏和骨骼肌衰老的基因表达谱研究。

Gene expression profiling studies of aging in cardiac and skeletal muscles.

作者信息

Park Sang-Kyu, Prolla Tomas A

机构信息

Department of Genetics and Medical Genetics, University of Wisconsin, Madison, WI 53706, USA.

出版信息

Cardiovasc Res. 2005 May 1;66(2):205-12. doi: 10.1016/j.cardiores.2005.01.005.

DOI:10.1016/j.cardiores.2005.01.005

PMID:15820189

Abstract

To examine transcriptional alterations associated with aging in skeletal muscle and the heart, we and others have used DNA microarrays to compare the gene expression profile of young and old animals. Aging results in a differential gene expression pattern specific to each tissue, and most alterations can be completely or partially prevented by caloric restriction (CR) in both heart and skeletal muscle. Transcriptional patterns of tissues from calorie-restricted animals suggests that CR retards the aging process by reducing endogenous damage and by inducing metabolic shifts associated with specific transcriptional profiles. These studies demonstrate that DNA microarrays can be used in cardiovascular aging research to generate panels of hundreds of transcriptional biomarkers, providing a new tool to measure biological age of cardiac and skeletal muscles and to test interventions designed to retard aging in these tissues.

摘要

为了研究骨骼肌和心脏中与衰老相关的转录变化，我们和其他研究人员使用DNA微阵列来比较年轻和年老动物的基因表达谱。衰老导致每个组织特有的差异基因表达模式，并且在心脏和骨骼肌中，大多数变化都可以通过热量限制（CR）完全或部分预防。来自热量限制动物的组织转录模式表明，CR通过减少内源性损伤和诱导与特定转录谱相关的代谢转变来延缓衰老过程。这些研究表明，DNA微阵列可用于心血管衰老研究，以生成数百种转录生物标志物的面板，为测量心脏和骨骼肌的生物学年龄以及测试旨在延缓这些组织衰老的干预措施提供了一种新工具。