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根癌土壤杆菌复杂基因组的联合遗传图谱和物理图谱

Combined genetic and physical map of the complex genome of Agrobacterium tumefaciens.

作者信息

Goodner B W, Markelz B P, Flanagan M C, Crowell C B, Racette J L, Schilling B A, Halfon L M, Mellors J S, Grabowski G

机构信息

Department of Biology, University of Richmond, Richmond, Virginia 23173, USA.

出版信息

J Bacteriol. 1999 Sep;181(17):5160-6. doi: 10.1128/JB.181.17.5160-5166.1999.

Abstract

A combined genetic and physical map of the Agrobacterium tumefaciens A348 (derivative of C58) genome was constructed to address the discrepancy between initial single-chromosome genetic maps and more recent physical mapping data supporting the presence of two nonhomologous chromosomes. The combined map confirms the two-chromosome genomic structure and the correspondence of the initial genetic maps to the circular chromosome. The linear chromosome is almost devoid of auxotrophic markers, which probably explains why it was missed by genetic mapping studies.

摘要

构建了根癌农杆菌A348(C58的衍生物)基因组的遗传图谱和物理图谱的组合,以解决最初的单染色体遗传图谱与最近支持存在两条非同源染色体的物理图谱数据之间的差异。组合图谱证实了双染色体基因组结构以及最初遗传图谱与环状染色体的对应关系。线性染色体几乎没有营养缺陷型标记,这可能解释了为什么它在遗传图谱研究中被遗漏。

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