Cheng L L, Rodas J D, Schultz K T, Christensen B M, Yuill T M, Israel B A
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 53706, USA.
Am J Trop Med Hyg. 1999 Mar;60(3):430-8. doi: 10.4269/ajtmh.1999.60.430.
Aedes albopictus was introduced into the United States in used tires in 1985. Its successful colonization of the upper Midwest has potential to alter the current epidemiology of bunyaviruses that circulate in the region. It is permissive for the replication of several arboviruses, including La Crosse (LACV) and Jamestown Canyon (JCV) bunyaviruses. In this study, we demonstrate the ability of LACV and JCV to coinfect Ae. albopictus mosquitoes and to form all six possible reassortant genotypes. All reassortant viruses infect Ae. albopictus orally and can be transmitted to suckling mice. All reassortants are neurovirulent in mice. However, reassortant viruses carrying the LACV M segment in the foreign genetic background of JCV are more neuroinvasive than JCV, or any other reassortant genotype. In addition, these reassortants can replicate in gerbils and infect Ae. triseriatus, characteristics of LACV, but not JCV. Because Ae. albopictus is spreading into new geographic areas and feeds on a variety of mammals, including humans, it has the potential to transmit new, emerging bunyaviruses in nature.
白纹伊蚊于1985年随废旧轮胎被引入美国。它在上中西部地区成功定殖，有可能改变该地区目前流行的布尼亚病毒的流行病学特征。它能允许包括拉克罗斯（LACV）和詹姆斯敦峡谷（JCV）布尼亚病毒在内的几种虫媒病毒复制。在本研究中，我们证明了LACV和JCV能够共同感染白纹伊蚊，并形成所有六种可能的重配基因型。所有重配病毒均可经口感染白纹伊蚊，并可传播给乳鼠。所有重配病毒在小鼠中均具有神经毒性。然而，在JCV的外源基因背景中携带LACV M节段的重配病毒比JCV或任何其他重配基因型更具神经侵袭性。此外，这些重配病毒能在沙鼠中复制并感染三带喙库蚊，这是LACV而非JCV的特征。由于白纹伊蚊正在扩散到新的地理区域，并以包括人类在内的多种哺乳动物为食，它有可能在自然界传播新出现的布尼亚病毒。
