Burkett D A, Kline D L, Carlson D A
Human System Center, Okinawa, Japan.
J Med Entomol. 1999 Jul;36(4):462-7. doi: 10.1093/jmedent/36.4.462.
Gas chromatography was used to analyze sugars found in individual crops fed on by wild caught adult Anopheles quadrimaculatus s.l. (Say), Coquillettidia perturbans (Walker), Culex nigripalpus Theobald, Culiseta melanura (Coquillett), and Psorophora ferox (von Humboldt) from several north central Florida locations. A wide range of sugars was found, including fructose, glucose, sucrose, maltose, turanose, melezitose, trehalose, raffinose, erlose, and traces of arabinose, rhamnose, and several unknowns. The frequency of individuals testing positive for crop sugars ranged from 9-10% in An. quadrimaculatus and Ps. ferox to 46% in Cq. perturbans. Based on the presence of melezitose or erlose in the crop, honeydew feeding was shown to be an important dietary component in An. quadrimaculatus (57%), Cs. melanura (31%), Cx. nigripalpus (15%), Cq. perturbans (10%), and Ps. ferox (7%). Gas chromatography of crude crop contents is a rapid one-step process that can address important questions such as the source and occurrence of sugar feeding in Diptera. Unique sugar feeding field observations were recorded for several mosquito species.
气相色谱法用于分析从佛罗里达州中北部几个地点捕获的野生成年四斑按蚊（Say）、骚扰库蚊（Walker）、黑须库蚊（Theobald）、黑尾脉毛蚊（Coquillett）和凶猛贪食蚊（von Humboldt）所取食的单个作物中发现的糖类。发现了多种糖类，包括果糖、葡萄糖、蔗糖、麦芽糖、松二糖、蜜三糖、海藻糖、棉子糖、糊精，以及微量的阿拉伯糖、鼠李糖和几种未知糖类。检测到作物糖类呈阳性的个体频率在四斑按蚊和凶猛贪食蚊中为9 - 10%，在骚扰库蚊中为46%。基于作物中蜜三糖或糊精的存在，取食蜜露被证明是四斑按蚊（57%）、黑尾脉毛蚊（31%）、黑须库蚊（15%）、骚扰库蚊（10%）和凶猛贪食蚊（7%）的重要饮食组成部分。对作物粗内容物进行气相色谱分析是一个快速的一步过程，可解决诸如双翅目昆虫取食糖类的来源和发生情况等重要问题。记录了几种蚊子独特的取食糖类野外观察结果。
