位于枯草芽孢杆菌主要谷氨酸脱氢酶基因下游的一个增强子元件。
An enhancer element located downstream of the major glutamate dehydrogenase gene of Bacillus subtilis.
作者信息
Belitsky B R, Sonenshein A L
机构信息
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USA.
出版信息
Proc Natl Acad Sci U S A. 1999 Aug 31;96(18):10290-5. doi: 10.1073/pnas.96.18.10290.
Abstract
The rocG gene of Bacillus subtilis, encoding a catabolic glutamate dehydrogenase, is transcribed by SigL (sigma(54))-containing RNA polymerase and requires for its expression RocR, a member of the NtrC/NifA family of proteins that bind to enhancer-like elements, called upstream activating sequences (UAS). Unlike the case for other sigma(54)-dependent genes, rocG has no UAS; instead, its expression depends on a sequence located 1.5 kilobases downstream of the rocG promoter, beyond the end of the rocG coding region. The same sequence also serves as the UAS for the downstream rocABC operon and can activate rocG if moved upstream of its promoter. Furthermore, the activating sequence can be moved as far as 15 kilobases downstream of the rocG promoter and still retain partial activity.
摘要
枯草芽孢杆菌的rocG基因编码一种分解代谢型谷氨酸脱氢酶，由含SigL（σ⁵⁴）的RNA聚合酶转录，其表达需要RocR，RocR是NtrC/NifA家族蛋白的成员，可与称为上游激活序列（UAS）的增强子样元件结合。与其他依赖σ⁵⁴的基因不同，rocG没有UAS；相反，其表达取决于位于rocG启动子下游1.5千碱基处的一个序列，该序列在rocG编码区末端之外。相同的序列还作为下游rocABC操纵子的UAS，如果移至rocG启动子上游，可激活rocG。此外，激活序列可移至rocG启动子下游多达15千碱基处，仍保留部分活性。
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