Saito S, Sakai M, Sasaki Y, Tanebe K, Tsuda H, Michimata T
Department of Obstetrics and Gynecology, Toyama Medical and Pharmaceutical University, Toyama, Japan.
Clin Exp Immunol. 1999 Sep;117(3):550-5. doi: 10.1046/j.1365-2249.1999.00997.x.
We calculated the percentage of Th1, Th2, Th0 cells and the Th1:Th2 cell ratio of peripheral blood from normal pregnant subjects and preeclampsia patients using flow cytometry which can analyse both the surface marker, CD4, and intracellular cytokines, interleukin (IL)-4 and interferon (IFN)-gamma. In normal pregnancy, the percentage of Th1 cells was significantly lower in the third trimester, and the ratios of Th1:Th2 were significantly lower in the second and third trimester than in nonpregnant subjects. In contrast, the percentage of Th1 cells and the ratios of Th1:Th2 in preeclampsia were significantly higher than in normal third trimester pregnant subjects. The percentage of Th2 cells in preeclampsia was significantly lower than in third trimester of normal pregnancy. Additionally, peripheral blood mononuclear cells from these subjects and patients were cultured with phytohemagglutinin stimulation, and IL-4 and IFN-gamma concentrations were determined in the supernatant by enzymed linked immunosorbent assays. The percentage of Th1 and Th2, and the ratios of Th1:Th2 were correlated with cytokine (IFN-gamma and IL-4) secretion level. These results demonstrated that Th2 cells were predominant in the second and third trimesters of normal pregnancy, but Th1 cells predominated in preeclamptic patients.
我们使用流式细胞术计算了正常孕妇和子痫前期患者外周血中Th1、Th2、Th0细胞的百分比以及Th1:Th2细胞比例，该技术可同时分析表面标志物CD4以及细胞内细胞因子白细胞介素（IL）-4和干扰素（IFN）-γ。在正常妊娠中，孕晚期Th1细胞百分比显著降低，孕中期和晚期Th1:Th2比例显著低于非孕女性。相比之下，子痫前期患者Th1细胞百分比和Th1:Th2比例显著高于正常孕晚期孕妇。子痫前期患者Th2细胞百分比显著低于正常妊娠晚期。此外，用植物血凝素刺激培养这些受试者和患者的外周血单个核细胞，通过酶联免疫吸附测定法测定上清液中IL-4和IFN-γ浓度。Th1和Th2细胞百分比以及Th1:Th2比例与细胞因子（IFN-γ和IL-4）分泌水平相关。这些结果表明，正常妊娠中期和晚期Th2细胞占主导，但子痫前期患者中Th1细胞占主导。
