Matthiesen L, Ekerfelt C, Berg G, Ernerudh J
Department of Obstetrics and Gynaecology, Faculty of Health Sciences, University of Linköping, Sweden.
Am J Reprod Immunol. 1998 Jun;39(6):362-7. doi: 10.1111/j.1600-0897.1998.tb00370.x.
Systemic immune responses during normal pregnancy are suggested to deviate toward secretion of T helper (Th)2-like cytokines.
Blood samples from 11 healthy primigravidae in their first, second, and third trimesters, and 8 weeks postpartum were examined. Peripheral blood mononuclear cells were separated and the numbers of interferon (IFN)-gamma- and interleukin (IL)-4-secreting cells were identified by using the enzyme-linked immunospot test.
In all three trimesters of pregnancy, and also postpartum, the numbers of IFN-gamma- and IL-4-secreting cells were significantly higher compared with nonpregnant controls (Mann-Whitney; P < 0.001). The numbers of IFN-gamma- and IL-4-secreting cells gradually increased as the pregnancy progressed compared with postpartum (Kruskas-Wallis; P < 0.01 and P < 0.05, for IFN-gamma and IL-4, respectively). The ratios of IFN-gamma/IL-4 during pregnancy and postpartum were unchanged, and also when compared with nonpregnant controls.
The results indicate a systemic up-regulation of both Th1- and Th2-like immune responses during normal pregnancy.
正常孕期的全身免疫反应倾向于向分泌辅助性T细胞（Th）2类细胞因子转变。
对11名处于孕早期、中期和晚期以及产后8周的健康初产妇的血样进行检测。分离外周血单核细胞，采用酶联免疫斑点试验鉴定分泌干扰素（IFN）-γ和白细胞介素（IL）-4的细胞数量。
与未孕对照组相比，孕期所有三个阶段及产后，分泌IFN-γ和IL-4的细胞数量均显著更高（曼-惠特尼检验；P<0.001）。与产后相比，随着孕期进展，分泌IFN-γ和IL-4的细胞数量逐渐增加（克鲁斯卡尔-沃利斯检验；IFN-γ和IL-4分别为P<0.01和P<0.05）。孕期和产后以及与未孕对照组相比时，IFN-γ/IL-4的比值均未改变。
结果表明正常孕期Th1类和Th2类免疫反应均出现全身上调。
