Saito S, Umekage H, Sakamoto Y, Sakai M, Tanebe K, Sasaki Y, Morikawa H
Department of Obstetrics and Gynecology, Toyama Medical and Pharmaceutical University, Japan.
Am J Reprod Immunol. 1999 May;41(5):297-306. doi: 10.1111/j.1600-0897.1999.tb00442.x.
To examine whether preeclampsia involves type-1 T-helper (TH1) immune hyperactivity.
Expression of HLA-DR, a cell-surface marker of activation, was analyzed on CD3+, CD4+, and CD8+ T cells in 15 preeclamptic patients and 15 normal pregnant women using flow cytometry. Additionally, peripheral blood mononuclear cells from preeclamptic patients and normal pregnant women were cultured with or without phytohemagglutinin (PHA) stimulation, and interleukin (IL)-2, IL-4, interferon (IFN)-gamma, and tumor necrosis factor (TNF)-alpha concentrations were determined in the supernatant by immunoassays.
HLA-DR antigen was expressed more strongly on CD3+ T cells in preeclamptic patients than in normal subjects. In preeclampsia, HLA-DR was expressed more strongly in CD8+ T cells than in CD4- T cells. More TNF-alpha, IL-2, and IFN-gamma were produced by unstimulated and stimulated cultured peripheral blood mononuclear cells from preeclampsia patients than by those from normal subjects. TNF-alpha/IL-4, IL-2/IL-4, and IFN-gamma/IL-4 ratios were higher in preeclamptic patients than in the normal group. Significant positive correlations were observed between mean blood pressure and concentrations of the Th-1 type cytokines IL-2, IFN-gamma, and TNF-alpha.
Up-regulation of Th1 responses and down-regulation of Th2 responses occur in preeclampsia.
研究子痫前期是否涉及1型辅助性T细胞（TH1）免疫功能亢进。
采用流式细胞术分析15例子痫前期患者和15例正常孕妇的CD3⁺、CD4⁺和CD8⁺T细胞上活化的细胞表面标志物HLA-DR的表达。此外，将子痫前期患者和正常孕妇的外周血单个核细胞在有或无植物血凝素（PHA）刺激的情况下进行培养，通过免疫测定法测定上清液中白细胞介素（IL）-2、IL-4、干扰素（IFN）-γ和肿瘤坏死因子（TNF）-α的浓度。
子痫前期患者CD3⁺T细胞上HLA-DR抗原的表达比正常受试者更强。在子痫前期，HLA-DR在CD8⁺T细胞中的表达比在CD4⁻T细胞中更强。子痫前期患者未刺激和刺激培养的外周血单个核细胞产生的TNF-α、IL-2和IFN-γ比正常受试者更多。子痫前期患者的TNF-α/IL-4、IL-2/IL-4和IFN-γ/IL-4比值高于正常组。平均血压与Th1型细胞因子IL-2、IFN-γ和TNF-α的浓度之间存在显著正相关。
子痫前期存在Th1反应上调和Th2反应下调。
