Michael K L, Walt D R
Department of Chemistry, Tufts University, Medford, Massachusetts 02155, USA.
Anal Biochem. 1999 Sep 10;273(2):168-78. doi: 10.1006/abio.1999.4173.
We demonstrate a microarray sensor capable of obtaining both chemical and visual information on multiple cells simultaneously with single-cell resolution. The array was fabricated by covalently immobilizing a thin, pH-sensitive polymer layer on the distal end of an optical imaging fiber. The sensor's ability to measure localized chemical dynamics in real-time was evaluated using sea urchin fertilization biochemistry as a model system. Following sea urchin fertilization, the Na(+)/H(+) transporter is activated to exchange extracellular sodium ions for intracellular hydrogen ions, causing a release of hydrogen ions at the egg's surface. By placing the pH sensor proximal to the egg and switching between a fluorescence image and a white light image, we were able to observe both localized pH changes following fertilization as well as morphological transformations during cell division.
我们展示了一种微阵列传感器，它能够以单细胞分辨率同时获取多个细胞的化学和视觉信息。该阵列是通过将一层薄的、对pH敏感的聚合物层共价固定在光学成像光纤的远端制成的。以海胆受精生物化学作为模型系统，评估了该传感器实时测量局部化学动力学的能力。在海胆受精后，Na(+)/H(+)转运体被激活，将细胞外钠离子与细胞内氢离子交换，导致卵子表面氢离子释放。通过将pH传感器放置在卵子附近，并在荧光图像和白光图像之间切换，我们能够观察到受精后局部pH值的变化以及细胞分裂过程中的形态转变。
