Tashima L S, Millar L K, Bryant-Greenwood G D
Pacific Biomedical Research Center, University of Hawaii, Honolulu, USA.
Obstet Gynecol. 1999 Sep;94(3):441-9. doi: 10.1016/s0029-7844(99)00339-7.
To determine whether suppression subtractive hybridization can detect genes in fetal membranes that are upregulated by infection, preterm premature rupture of membranes (PROM), or labor.
Using suppression subtractive hybridization, messenger RNAs from a preterm fetal membrane obtained at cesarean delivery without labor (control) were subtracted from a pool of messenger RNAs of three patients with preterm PROM and vaginal delivery. Eight candidate genes identified as upregulated were quantitated by Northern analysis in each of the tissues and in additional patient subgroups.
Eight differentially upregulated genes were identified in preterm labor with PROM. Four of the genes are known to be involved in the response to inflammation or infection, and subsequent histologic examination showed one of the preterm PROM tissues to be infected. F-actin capping protein and chitinase precursor, not previously known to be involved in infection, were also upregulated in the infected tissue from preterm PROM. Northern blots using additional subgroups of patients showed that a regulatory G-protein signaling protein gene was significantly upregulated at term by labor in addition to significant upregulation of interleukin-8. There was a strong correlation between the gene expression for complement factor-B and duration of membrane rupture in the patients with preterm PROM.
Two novel genes potentially involved in the response to inflammation or infection have been identified. A regulatory G-protein signaling protein and interleukin-8 gene expression were upregulated by labor. Complement factor-B gene expression was directly related to the duration of membrane rupture.
确定抑制性消减杂交技术能否检测出胎膜中因感染、早产胎膜早破（PROM）或分娩而上调的基因。
采用抑制性消减杂交技术，从剖宫产未临产（对照）的早产胎膜中提取的信使核糖核酸（mRNA），与3例早产胎膜早破经阴道分娩患者的mRNA混合样本进行消减。通过Northern印迹分析对鉴定为上调的8个候选基因在每个组织及其他患者亚组中进行定量分析。
在早产胎膜早破的早产分娩中鉴定出8个差异上调基因。其中4个基因已知参与炎症或感染反应，随后的组织学检查显示1例早产胎膜早破组织被感染。F-肌动蛋白封端蛋白和几丁质酶前体，此前未知与感染有关，在早产胎膜早破的感染组织中也上调。对其他患者亚组进行的Northern印迹分析显示，除白细胞介素-8显著上调外，一种调节性G蛋白信号蛋白基因在足月分娩时也显著上调。在早产胎膜早破患者中，补体因子B的基因表达与胎膜破裂持续时间密切相关。
已鉴定出两个可能参与炎症或感染反应的新基因。一种调节性G蛋白信号蛋白和白细胞介素-8基因表达在分娩时上调。补体因子B基因表达与胎膜破裂持续时间直接相关。
