Sahm K, Knoblauch C, Amann R
Molecular Ecology Group, Max-Planck-Institute for Marine Microbiology, D-28359 Bremen, Germany.
Appl Environ Microbiol. 1999 Sep;65(9):3976-81. doi: 10.1128/AEM.65.9.3976-3981.1999.
Thirteen psychrophilic sulfate-reducing isolates from two permanently cold fjords of the Arctic island Spitsbergen (Hornsund and Storfjord) were phylogenetically analyzed. They all belonged to the delta subclass of Proteobacteria and were widely distributed within this group, indicating that psychrophily is a polyphyletic property. A new 16S rRNA-directed oligonucleotide probe was designed against the largest coherent cluster of these isolates. The new probe, as well as a set of available probes, was applied in rRNA slot blot hybridization to investigate the composition of the sulfate-reducing bacterial community in the sediments. rRNA related to the new cluster of incompletely oxidizing, psychrophilic isolates made up 1.4 to 20.9% of eubacterial rRNA at Storfjord and 0.6 to 3. 5% of eubacterial rRNA at Hornsund. This group was the second-most-abundant group of sulfate reducers at these sites. Denaturing gradient gel electrophoresis and hybridization analysis showed bands identical to those produced by our isolates. The data indicate that the psychrophilic isolates are quantitatively important in Svalbard sediments.
对从北极斯瓦尔巴群岛的两个常年寒冷峡湾（霍恩松德和大峡湾）分离出的13株嗜冷硫酸盐还原菌进行了系统发育分析。它们都属于变形菌纲的δ亚纲，并且在该类群中分布广泛，这表明嗜冷性是一种多系属性。针对这些分离株中最大的连贯聚类设计了一种新的16S rRNA导向的寡核苷酸探针。将新探针以及一组现有的探针应用于rRNA斑点杂交，以研究沉积物中硫酸盐还原细菌群落的组成。与不完全氧化的嗜冷分离株新聚类相关的rRNA在大峡湾占真细菌rRNA的1.4%至20.9%，在霍恩松德占真细菌rRNA的0.6%至3.5%。该类群是这些地点第二丰富的硫酸盐还原菌群。变性梯度凝胶电泳和杂交分析显示的条带与我们的分离株产生的条带相同。数据表明，嗜冷分离株在斯瓦尔巴群岛的沉积物中数量上很重要。
