Vemulapalli R, McQuiston J R, Schurig G G, Sriranganathan N, Halling S M, Boyle S M
Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg 24061-0342, USA.
Clin Diagn Lab Immunol. 1999 Sep;6(5):760-4. doi: 10.1128/CDLI.6.5.760-764.1999.
Brucella abortus vaccine strain RB51 is a natural stable attenuated rough mutant derived from the virulent strain 2308. The genetic mutations that are responsible for the roughness and the attenuation of strain RB51 have not been identified until now. Also, except for an assay based on pulsed-field gel electrophoresis, no other simple method to differentiate strain RB51 from its parent strain 2308 is available. In the present study, we demonstrate that the wboA gene encoding a glycosyltransferase, an enzyme essential for the synthesis of O antigen, is disrupted by an IS711 element in B. abortus vaccine strain RB51. Exploiting this feature, we developed a PCR assay that distinguishes strain RB51 from all other Brucella species and strains tested.
布鲁氏菌流产疫苗株RB51是一种从强毒株2308衍生而来的天然稳定减毒粗糙突变株。迄今为止，尚未确定导致RB51菌株粗糙性和减毒的基因突变。此外，除了基于脉冲场凝胶电泳的检测方法外，没有其他简单方法可用于区分RB51菌株与其亲本菌株2308。在本研究中，我们证明编码糖基转移酶（一种O抗原合成所必需的酶）的wboA基因在布鲁氏菌流产疫苗株RB51中被一个IS711元件破坏。利用这一特性，我们开发了一种PCR检测方法，可将RB51菌株与所有其他测试的布鲁氏菌属物种和菌株区分开来。
