用于区分流产布鲁氏菌疫苗株S19和RB51的布鲁氏菌AMOS PCR检测方法的改进
Enhancement of the Brucella AMOS PCR assay for differentiation of Brucella abortus vaccine strains S19 and RB51.
作者信息
Bricker B J, Halling S M
机构信息
National Animal Disease Center, Agricultural Research Service, Ames, Iowa 50010, USA.
出版信息
J Clin Microbiol. 1995 Jun;33(6):1640-2. doi: 10.1128/jcm.33.6.1640-1642.1995.
Abstract
Because the brucellosis eradication program uses slaughter and quarantine as control measures, it would benefit from faster methods of bacterial identification. Distinguishing vaccine strains from strains that cause infections among vaccinated herds in the field is essential. To accomplish this, our PCR-based, species-specific assay (B. J. Bricker and S. M. Halling, J. Clin. Microbiol. 32:2660-2666, 1994) was updated to identify Brucella abortus vaccine strains S19 and RB51. Three new oligonucleotide primers were added to the five-primer multiplex Brucella AMOS PCR assay. Identification is based on the number and sizes of six products amplified by PCR.
摘要
由于布鲁氏菌病根除计划采用屠宰和检疫作为控制措施，因此更快的细菌鉴定方法将对其有所助益。区分疫苗菌株与在野外接种牛群中引起感染的菌株至关重要。为实现这一目标，我们对基于PCR的种特异性检测方法（B. J. 布里克和S. M. 哈林，《临床微生物学杂志》32:2660 - 2666，1994年）进行了更新，以鉴定布鲁氏菌流产疫苗菌株S19和RB51。在五引物多重布鲁氏菌AMOS PCR检测方法中添加了三种新的寡核苷酸引物。鉴定基于PCR扩增的六种产物的数量和大小。
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