Campbell W P, Huang C
Wadsworth Center, New York State Department of Health, Albany 12201-0509, USA.
Virus Res. 1999 Jun;61(2):137-44. doi: 10.1016/s0168-1702(99)00033-7.
The complete nucleotide sequences have been determined for the M segment of 12 California (CAL) serogroup bunyaviruses. A method is described here of long reverse transcription-polymerase chain reaction (RT-PCR) that yields the full-length medium (M) RNA genomic segment. A phylogenetic tree was constructed by comparison of the open reading frames (ORFs) in the M RNA segment of 15 CAL serogroup viruses. Three distinct branches were identified and they are represented by the California encephalitis (CE), Melao (MEL), and Trivittatus (TVT) complexes. These groups correspond to those previously established by small (S) RNA genomic sequences. In addition, except for Inkoo virus, the predicted relationship among these viruses agreed with those found by serology.
已确定12种加利福尼亚（CAL）血清群布尼亚病毒M节段的完整核苷酸序列。本文描述了一种长链逆转录-聚合酶链反应（RT-PCR）方法，该方法可产生全长中（M）RNA基因组节段。通过比较15种CAL血清群病毒M RNA节段中的开放阅读框（ORF）构建了系统发育树。鉴定出三个不同的分支，它们分别由加利福尼亚脑炎（CE）、梅劳（MEL）和三带喙库蚊（TVT）复合体代表。这些组与先前通过小（S）RNA基因组序列建立的组相对应。此外，除因库奥病毒外，这些病毒之间的预测关系与血清学结果一致。
