Vapalahti O, Plyusnin A, Cheng Y, Manni T, Brummer-Korvenkontio M, Vaheri A
Department of Virology, University of Helsinki, Finland.
J Gen Virol. 1996 Aug;77 ( Pt 8):1769-74. doi: 10.1099/0022-1317-77-8-1769.
Inkoo (INK) and Tahyna (TAH) viruses, European representatives of the California serogroup (CAL), genus Bunyavirus, family Bunyaviridae, are transmitted by mosquitoes and frequently infect man. The S segments of INK and TAH prototype strains were amplified, cloned and sequenced. INK S consists of 986 and TAH S of 977 nucleotides (nt) coding for a nucleocapsid protein of 235 amino acids (aa) and, in an overlapping reading frame, for a nonstructural protein of 92 or 97 aa, respectively. By S segment sequences and phylogenetic analysis INK was seen to be most closely related to Jamestown Canyon virus, isolated in the USA (92.4% nt and 96.6% aa identity), which is currently classified in a different subcomplex within the CAL viruses. TAH was genetically closest to Lumbo virus, isolated in Mozambique (89.0% nt and 94.1% aa identity). The data suggest that genetic variation within the CAL viruses is less related to geographical distance than to similarity in ecological cycles.
因库奥（INK）病毒和塔伊纳（TAH）病毒是布尼亚病毒科布尼亚病毒属加利福尼亚血清群（CAL）的欧洲代表病毒，通过蚊子传播，且经常感染人类。对INK和TAH原型毒株的S片段进行了扩增、克隆和测序。INK的S片段由986个核苷酸组成，TAH的S片段由977个核苷酸组成，分别编码一个235个氨基酸的核衣壳蛋白，并且在一个重叠阅读框中分别编码一个92或97个氨基酸的非结构蛋白。通过S片段序列和系统发育分析发现，INK与在美国分离出的詹姆斯敦峡谷病毒关系最为密切（核苷酸同一性为92.4%，氨基酸同一性为96.6%），该病毒目前被归类于CAL病毒内的一个不同亚群。TAH在基因上与在莫桑比克分离出的伦博病毒最为接近（核苷酸同一性为89.0%，氨基酸同一性为94.1%）。数据表明，CAL病毒内的遗传变异与地理距离的关系小于与生态循环相似性的关系。
