Huang C, Shope R E, Spargo B, Campbell W P
Wadsworth Center, New York State Department of Health, Albany 12201-0509, USA. Cinnia
J Gen Virol. 1996 Aug;77 ( Pt 8):1761-8. doi: 10.1099/0022-1317-77-8-1761.
The complete nucleotide sequences of the small (S) genomic RNA segments of five California (CAL) serogroup bunyaviruses (two Inkoo virus strains, San Angelo virus, Serra do Navio virus, South River virus and Tahyna virus) were determined. In agreement with previously published data concerning CAL serogroup viruses, the nucleocapsid (N) and non-structural (NSs) proteins were encoded in over-lapping open reading frames (ORFs). All N protein ORFs were 708 nucleotides in length and encoded a 235 amino-acid gene product. The NSs ORFs were either 279 or 294 nucleotides in length, which would encode 92 or 97 amino-acid proteins, respectively. Comparative analysis of the nucleotide sequences and amino acids corresponding to the nucleocapsid protein resulted in a predicted relationship among these viruses that generally agreed with those determined by serology. The only exception was Inkoo virus, where comparisons based on the S RNA sequence and partial M RNA sequence suggest that this virus is more similar to Jamestown Canyon virus of the Melao complex than it is to viruses such as Tahyna and La Crosse viruses of the California encephalitis complex.
测定了五种加利福尼亚（CAL）血清群布尼亚病毒（两种因库奥病毒株、圣安杰洛病毒、塞拉多纳维奥病毒、南河病毒和塔伊纳病毒）小（S）基因组RNA片段的完整核苷酸序列。与先前发表的有关CAL血清群病毒的数据一致，核衣壳（N）蛋白和非结构（NSs）蛋白由重叠开放阅读框（ORF）编码。所有N蛋白ORF长度均为708个核苷酸，编码一个235个氨基酸的基因产物。NSs ORF长度分别为279或294个核苷酸，分别编码92或97个氨基酸的蛋白质。对与核衣壳蛋白对应的核苷酸序列和氨基酸进行比较分析，得出了这些病毒之间的预测关系，这与血清学确定的关系总体一致。唯一的例外是因库奥病毒，基于S RNA序列和部分M RNA序列的比较表明，该病毒与梅劳复合体的詹姆斯敦峡谷病毒比与加利福尼亚脑炎复合体的塔伊纳病毒和拉克罗斯病毒等病毒更相似。
