Zhu C Q, Lam T H, Jiang C Q, Wei B X, Lou X, Liu W W, Lao X Q, Chen Y H
Guangzhou Occupational Diseases Prevention and Treatment Center, Huang Po Dong, Bai Yun Mountain, Guangzhou, China.
Mutat Res. 1999 Jul 21;444(1):1-6. doi: 10.1016/s1383-5718(99)00097-2.
To investigate whether there were separate and combined effects of occupational exposure to tobacco dust and smoking on lymphocyte DNA damage, 148 workers from a cigarette manufacturing factory (107 occupationally exposed to tobacco dust from the production department and 41 unexposed controls who were managerial workers) were included in the study. The Tail Moment (TM) of Comet assay was used to measure DNA damage. The two groups had similar mean age, mean duration of work and smoking prevalence. The exposed workers had a larger TM than that of the controls (mean+/-S.D.: 43.43+/-13. 77 vs. 38.89+/-8.98, p<0.05). Smokers had significantly larger TM than non-smokers (47.25+/-14.02 vs. 38.90+/-10.75, p<0.001). Analysis of variance after adjustment for age and gender showed that occupational exposure and smoking had a significant and independent effect on Tail Moment (p=0.025 and p=0.002, respectively) and there was a significant positive two way interaction between the two factors (p=0.019). Age and gender had no significant effect on TM. The present study suggests that smoking and tobacco dust exposure can induce lymphocyte DNA damage and there is a synergistic effect of tobacco dust exposure and smoking on DNA damage.
为了研究职业性接触烟草粉尘和吸烟对淋巴细胞DNA损伤的单独及联合影响，本研究纳入了一家卷烟厂的148名工人（其中107名来自生产部门，职业性接触烟草粉尘，41名未接触烟草粉尘的管理人员作为对照）。采用彗星试验的尾矩（TM）来测量DNA损伤。两组在平均年龄、平均工作年限和吸烟率方面相似。接触烟草粉尘的工人的TM值高于对照组（平均值±标准差：43.43±13.77 vs. 38.89±8.98，p<0.05）。吸烟者的TM值显著高于非吸烟者（47.25±14.02 vs. 38.90±10.75，p<0.001）。在对年龄和性别进行调整后的方差分析显示，职业性接触和吸烟对尾矩有显著且独立的影响（分别为p=0.025和p=0.002），并且这两个因素之间存在显著的正向双向交互作用（p=0.019）。年龄和性别对TM没有显著影响。本研究表明，吸烟和接触烟草粉尘均可诱导淋巴细胞DNA损伤，且接触烟草粉尘和吸烟在DNA损伤方面存在协同作用。
