使用3个彗星试验参数对职业性接触铅的工人的DNA损伤进行评估。
Evaluation of DNA damage using 3 comet assay parameters in workers occupationally exposed to lead.
作者信息
Kayaaltı Zelıha, Yavuz İlknur, Söylemez Esma, Bacaksız Ayşegül, Tutkun Engın, Sayal Ahmet, Söylemezoğlu Tülın
机构信息
a Institute of Forensic Sciences, Ankara University , Ankara , Turkey.
出版信息
Arch Environ Occup Health. 2015;70(3):120-5. doi: 10.1080/19338244.2013.787964.
The aim of this study was to investigate the association between DNA damage and blood lead levels in individuals occupationally exposed to lead. To evaluate this association, 61 workers exposed to lead were monitored in terms of DNA damage in blood lymphocytes. The levels of DNA damage were measured according to 3 comet assay parameters, including tail intensity (TI), tail moment (TM), and DNA tail (DNAt). A statistically significant positive correlation was found between the lead levels and TI, TM, and DNAt (p < .01). Smoking had independent effects on DNA damage. A statistically significant difference was observed between smokers and nonsmokers in regards to DNA damage parameters (p < .05). In addition, the lead and DNA damage levels in smokers were found to be significantly higher than the levels observed in nonsmoking workers (p < . 05). Our results show that exposure to lead induces genotoxic effects in peripheral lymphocytes, as measured by comet assays.
本研究的目的是调查职业性接触铅的个体中DNA损伤与血铅水平之间的关联。为评估这种关联，对61名接触铅的工人的血液淋巴细胞DNA损伤情况进行了监测。根据彗星试验的3个参数测量DNA损伤水平，包括尾强度（TI）、尾矩（TM）和DNA尾（DNAt）。发现铅水平与TI、TM和DNAt之间存在统计学上显著的正相关（p < 0.01）。吸烟对DNA损伤有独立影响。在DNA损伤参数方面，吸烟者与非吸烟者之间观察到统计学上显著的差异（p < 0.05）。此外，发现吸烟者的铅和DNA损伤水平显著高于非吸烟工人的观察水平（p < 0.05）。我们的结果表明，通过彗星试验测量，接触铅会在外周淋巴细胞中诱导遗传毒性效应。
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