卵泡发育和成熟对人绒毛膜促性腺激素和重组人促黄体生成素的需求。
Requirements for human chorionic gonadotropin and recombinant human luteinizing hormone for follicular development and maturation.
作者信息
Andersen C Y, Ziebe S, Guoliang X, Byskov A G
机构信息
Laboratory of Reproductive Biology, Juliane Marie Center for Children, Women and Reproduction, University Hospital of Copenhagen, Denmark.
出版信息
J Assist Reprod Genet. 1999 Sep;16(8):425-30. doi: 10.1023/a:1020569508927.
Abstract
PURPOSE
Our purpose was to evaluate the requirements for human chorionic gonadotropin (hCG) and recombinant luteinizing hormone (rec-LH) for follicular development and maturation in mice.
METHODS
We carried out ovarian stimulation of immature mice. Output parameters were the preembryos created in vivo and frequency of blastocyst formation in vitro.
RESULTS
hCG at 0 to 1 IU resulted in a dose-dependent recovery of preembryos (0 to 39.7 +/- 4.3; mean +/- SE) per mouse. hCG at 1 and 10 hCG gave similar results, whereas higher doses significantly reduced the number of preembryos. Potential for blastocyst formation was independent of hCG dose. hCG and rec-LH together exerted a synergistic effect on the recovery of preembryos.
CONCLUSIONS
Optimal follicular development required a combination of 20 IU follicle stimulating hormone and 1-10 IU hCG. The potency of hCG was higher than that of rec-LH, but a synergistic effect of rec-LH and hCG was observed. The results may be pertinent for the development of strategies for ovarian stimulation of women with low levels of endogenous LH.
摘要
目的
我们的目的是评估小鼠卵泡发育和成熟过程中对人绒毛膜促性腺激素（hCG）和重组促黄体生成素（rec-LH）的需求。
方法
我们对未成熟小鼠进行卵巢刺激。输出参数为体内产生的前胚胎和体外囊胚形成频率。
结果
0至1 IU的hCG导致每只小鼠的前胚胎数量呈剂量依赖性恢复（0至39.7 +/- 4.3；平均值 +/- 标准误）。1 IU和10 IU的hCG产生相似结果，而更高剂量则显著减少前胚胎数量。囊胚形成潜力与hCG剂量无关。hCG和rec-LH共同对前胚胎的恢复发挥协同作用。
结论
最佳卵泡发育需要20 IU促卵泡激素和1 - 10 IU hCG的组合。hCG的效力高于rec-LH，但观察到rec-LH和hCG有协同作用。这些结果可能与制定针对内源性LH水平低的女性的卵巢刺激策略相关。
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