Uotinen N, Puustinen R, Pasanen S, Manninen T, Kivineva M, Syvälä H, Tuohimaa P, Ylikomi T
Medical School, University of Tampere, Tampere, FIN-33101, Finland.
Gen Comp Endocrinol. 1999 Sep;115(3):429-41. doi: 10.1006/gcen.1999.7333.
Two novel antibodies against the mammalian progesterone receptor (PR) were raised and characterized to study the distribution of PR and the effect of estrogen on PR expression in various female murine tissues by immunohistochemistry. There were estrogen-independent constitutive PR expressions in the smooth muscle cells of uterus, uterine blood vessels, urinary bladder, duodenum, and jejunum of ovariectomized mice. Uterine stromal cells, capsular cells of kidney and adrenal gland, and the epithelial cells of submandibular gland expressed PR constitutively. PR expression was detected in some thymic cells and the number of PR-positive thymic cells increased markedly after estrogen treatment. Estrogen induced PR expression in the epithelial cells of uterus, vagina, urethra, and skin and the stromal cells of vagina, urethra, and pancreatic ducts, as well as the smooth muscle cells of some blood vessels. These results suggest cell-specific progesterone actions in the urinary tract, skin, and gastrointestinal organs, on the immune functions, and on the regulation of local blood flow.
制备并鉴定了两种针对哺乳动物孕酮受体（PR）的新型抗体，以通过免疫组织化学研究PR的分布以及雌激素对各种雌性小鼠组织中PR表达的影响。在去卵巢小鼠的子宫平滑肌细胞、子宫血管、膀胱、十二指肠和空肠中存在不依赖雌激素的组成性PR表达。子宫基质细胞、肾和肾上腺的被膜细胞以及下颌下腺的上皮细胞组成性表达PR。在一些胸腺细胞中检测到PR表达，雌激素处理后PR阳性胸腺细胞数量明显增加。雌激素诱导子宫、阴道、尿道和皮肤的上皮细胞以及阴道、尿道和胰管的基质细胞以及一些血管的平滑肌细胞中PR表达。这些结果表明孕酮在泌尿道、皮肤和胃肠道器官、免疫功能以及局部血流调节中具有细胞特异性作用。
