眼脉动性对扫描激光多普勒血流仪的影响。

The influence of ocular pulsatility on scanning laser Doppler flowmetry.

作者信息

Sullivan P, Cioffi G A, Wang L, Johnson C A, Van Buskirk E M, Sherman K R, Bacon D R

机构信息

Discoveries in Sight, Devers Eye Institute, Portland, Oregon, USA.

出版信息

Am J Ophthalmol. 1999 Jul;128(1):81-7. doi: 10.1016/s0002-9394(99)00072-0.

DOI:10.1016/s0002-9394(99)00072-0

PMID:10482098

Abstract

PURPOSE

To determine the effect of the cardiac cycle on scanning laser Doppler flowmeter measurements of retinal capillary blood flow in rhesus monkeys and humans.

METHODS

Multiple scanning laser Doppler flowmetry images of rhesus monkey and human retinal capillary blood flow over a range of heart rates were obtained. Average flow values were determined for the 64 scan lines that compose the two-dimensional flow map. Cutaneous blood flow was measured simultaneously with a laser Doppler flowmeter. The temporal relationships between retinal capillary blood flow, peripheral arterial pulse, and cutaneous blood flow were determined. In addition, human retinal capillary blood flow in a 10 x 10-pixel area during different phases of the cardiac cycle was compared.

RESULTS

Regular oscillations in human and rhesus monkey retinal capillary blood flow are evident as alternating bright and dark horizontal bands in scanning laser Doppler flowmetry images. These fluctuations are temporally correlated with cutaneous blood flow. Linear regression of actual vs predicted heart rate based on peaks in retinal capillary flow yielded r = 0.999 in a rhesus monkey and 0.938 in a human. Retinal capillary blood flow in a 10 x 10-pixel area fluctuated as much as 50% depending on the phase of the cardiac cycle.

CONCLUSIONS

The alternating bright and dark banding pattern observed in scanning laser Doppler flowmetry scans of retinal capillary blood flow is related to the cardiac pulse. The errors introduced by pulse-related fluctuations in retinal capillary blood flow are significant and must be minimized or corrected for accurate and reproducible measurements of ocular hemodynamics.

摘要

目的

确定心动周期对恒河猴和人类视网膜毛细血管血流的扫描激光多普勒流量计测量结果的影响。

方法

获取了一系列心率下恒河猴和人类视网膜毛细血管血流的多个扫描激光多普勒血流图图像。确定构成二维血流图的64条扫描线的平均血流值。同时用激光多普勒流量计测量皮肤血流。确定视网膜毛细血管血流、外周动脉脉搏和皮肤血流之间的时间关系。此外，还比较了心动周期不同阶段10×10像素区域内的人类视网膜毛细血管血流。

结果

在扫描激光多普勒血流图图像中，人类和恒河猴视网膜毛细血管血流的规则振荡表现为交替出现的明亮和黑暗水平带。这些波动与皮肤血流在时间上相关。基于视网膜毛细血管血流峰值对实际心率与预测心率进行线性回归，在恒河猴中r = 0.999，在人类中r = 0.938。根据心动周期的阶段，10×10像素区域内的视网膜毛细血管血流波动高达50%。