Wu P Z, Zhu H, Thakur A, Willcox M D
Cooperative Research Centre for Eye Research and Technology, School of Optometry, University of New South Wales, Sydney, Australia.
Aust N Z J Ophthalmol. 1999 Jun-Aug;27(3-4):234-6. doi: 10.1046/j.1440-1606.1999.00192.x.
Staphylococcus epidermidis and Staphylococcus aureus are two of the commonest bacteria isolated from corneal ulcers. The aim of the current investigation was to determine the frequency of potentially pathogenic traits in the two staphylococcal species. Strains of both species, some isolated from eyes during active corneal inflammation, were screened for their ability to degrade a variety of proteins and hyaluronic acid and the production of cytotoxins. S. aureus produced more tissue-destructive enzymes than the S. epidermidis. S. aureus strains more commonly synthesized the cytotoxin, alpha-toxin. The production of elastase was more common among S. aureus strains isolated from ulcerative events. The production of alpha-toxin was inversely correlated with isolation from keratitis. This study has demonstrated that S. aureus is better equipped with a range of potentially damaging enzymes and toxins than S. epidermidis and that S. aureus elastase may be involved in the production of corneal ulcers.
表皮葡萄球菌和金黄色葡萄球菌是从角膜溃疡中分离出的两种最常见的细菌。本研究的目的是确定这两种葡萄球菌潜在致病特性的频率。对这两种菌种的菌株进行了筛选，其中一些菌株是在角膜炎症活跃期从眼睛中分离出来的，检测它们降解多种蛋白质和透明质酸的能力以及细胞毒素的产生情况。金黄色葡萄球菌产生的组织破坏性酶比表皮葡萄球菌更多。金黄色葡萄球菌菌株更常合成细胞毒素α毒素。在从溃疡性病变中分离出的金黄色葡萄球菌菌株中，弹性蛋白酶的产生更为常见。α毒素的产生与从角膜炎中分离呈负相关。这项研究表明，与表皮葡萄球菌相比，金黄色葡萄球菌具备一系列潜在破坏性酶和毒素的能力更强，并且金黄色葡萄球菌弹性蛋白酶可能参与角膜溃疡的产生。
