Esemu Seraphine Nkie, Nya'Nying Shantal Fuenbi, Ndip Lucy Mande, Bessong Pascal Obong, Tanih Nicoline Fri, Smith Stella Ifeanyi, Ndip Roland Ndip
Department of Microbiology and Parasitology, Faculty of Science, University of Buea, Box 63, Buea, Cameroon.
Laboratory for Emerging Infectious Diseases, University of Buea, Box 63, Buea, Cameroon.
BMC Res Notes. 2024 Dec 27;17(1):389. doi: 10.1186/s13104-024-07073-3.
Methicillin-resistant Staphylococcus aureus (MRSA) is a zoonotic pathogen that poses a serious threat to veterinary and public health worldwide. We investigated mastitis milk samples for contamination with MRSA and also characterized the MRSA isolates by investigating antimicrobial resistance and virulence factors.
We confirmed MRSA in 69 of 201 (34.3%) S. aureus isolates recovered from a total of 300 samples. Of the 69 MRSA, 19 (27.5%) were from subclinical cases, while 50 (72.5%) were from clinical cases. The MRSA showed high resistance to penicillin (100%), ampicillin (100%), trimethoprim (69.6%), and tetracycline (69.6%) while susceptibility was observed for gentamicin (100%), vancomycin (95.7%), and ciprofloxacin (91.3%). Most isolates (65.2%, 45/69) were multidrug resistant. Thirteen antibiotypes (A-A) were identified and the most prevalent was A (TMPETETAMPP). All MRSA produced haemolysins, caseinase, and coagulase. Lipase, gelatinase and lecithinase were found in 97.1%, 94.2% and 91.3% of isolates respectively. Genotyping revealed coa (100%) and spa (68.1%) genes. We recommend educating dairy farmers on the public health implications of consuming unpasteurized raw milk and the implementation of proper hygiene practices in dairy farms.
耐甲氧西林金黄色葡萄球菌（MRSA）是一种人畜共患病原体，对全球兽医和公共卫生构成严重威胁。我们调查了乳腺炎乳样中是否存在MRSA污染，并通过研究抗菌药物耐药性和毒力因子对MRSA分离株进行了特征分析。
我们从总共300份样本中回收的201株金黄色葡萄球菌分离株中，确认了69株（34.3%）为MRSA。在这69株MRSA中，19株（27.5%）来自亚临床病例，而50株（72.5%）来自临床病例。MRSA对青霉素（100%）、氨苄青霉素（100%）、甲氧苄啶（69.6%）和四环素（69.6%）表现出高度耐药性，而对庆大霉素（100%）、万古霉素（95.7%）和环丙沙星（91.3%）敏感。大多数分离株（65.2%，45/69）具有多重耐药性。鉴定出13种抗菌型（A - A），最常见的是A（TMPETETAMPP）。所有MRSA均产生溶血素、酪蛋白酶和凝固酶。分别在97.1%、94.2%和91.3%的分离株中发现了脂肪酶、明胶酶和卵磷脂酶。基因分型显示coa（100%）和spa（68.1%）基因。我们建议对奶农进行关于食用未巴氏杀菌生牛奶对公共卫生影响的教育，并在奶牛场实施适当的卫生措施。
