Lipps H J, Steinbrück G
Chromosoma. 1978 Oct 20;69(1):21-6. doi: 10.1007/BF00327378.
When separated on an agarose gel, macronuclear DNA of the hypotrichous ciliate Stylonychia mytilus gives rise to many well-defined bands ranging in molecular weight from 0.3 x 10(6) to 14 x 10(6) dalton. Hybridization of 25 S rRNA, 17S rRNA or 5 S RNA to such a gel revealed sharp hybridization bands. This suggests that this banding pattern is not an artefact due to nonspecific degradation of macronuclear DNA but that the DNA in the macronucleus of Stylonychia occurs in discrete fragments, each coding for at least one gene. The size of the DNA fragment coding for rRNA was found to be 4.5 x 10(6) dalton, the fragment coding for 5 S RNA has a molecular weight of 150,000-250,000 dalton.
当在琼脂糖凝胶上进行分离时，腹毛目纤毛虫贻贝棘尾虫的大核DNA会产生许多界限清晰的条带，分子量范围从0.3×10⁶到14×10⁶道尔顿。将25 S rRNA、17S rRNA或5 S RNA与这样的凝胶进行杂交，会显示出清晰的杂交条带。这表明这种条带模式并非由于大核DNA的非特异性降解造成的人为假象，而是贻贝棘尾虫大核中的DNA以离散片段的形式存在，每个片段至少编码一个基因。发现编码rRNA的DNA片段大小为4.5×10⁶道尔顿，编码5 S RNA的片段分子量为150,000 - 250,000道尔顿。
