Atyeo R F, Oxberry S L, Hampson D J
Division of Veterinary and Biomedical Sciences, Murdoch University, Western Australia, Australia.
Epidemiol Infect. 1999 Aug;123(1):133-8. doi: 10.1017/s0950268899002691.
Pulsed-field gel electrophoresis (PFGE) was applied as a molecular typing tool for the spirochaete Serpulina hyodysenteriae, the agent of swine dysentery. Analysis of a collection of 40 mainly Australian isolates, previously characterized by other methods, divided these into 23 PFGE types. This confirmed that there are many strains of the spirochaete in Australia. PFGE was more discriminatory for strain typing than both multilocus enzyme electrophoresis and serotyping. It had similar discriminatory power to restriction endonuclease analysis, but the results of PFGE were easier to interpret. When applied to 29 isolates collected from 4 farms over periods of up to 8 years, 2 PFGE patterns were found on 3 farms, and a single pattern on the other. In each case a new strain had apparently emerged as a variant of an original parent strain. PFGE was found to be a powerful technique for investigating the molecular epidemiology of swine dysentery outbreaks.
脉冲场凝胶电泳（PFGE）被用作猪痢疾病原体——猪痢疾蛇形螺旋体的分子分型工具。对40株主要来自澳大利亚的分离株（此前已用其他方法进行过鉴定）进行分析，将它们分为23种PFGE型。这证实了澳大利亚存在许多该螺旋体菌株。与多位点酶电泳和血清分型相比，PFGE在菌株分型上具有更高的鉴别力。它与限制性内切酶分析具有相似的鉴别力，但PFGE的结果更易于解释。将PFGE应用于从4个猪场收集的、长达8年期间的29株分离株时，在3个猪场发现了2种PFGE图谱，另一个猪场为单一图谱。在每种情况下，一个新菌株显然是从原始亲本菌株变异而来。结果发现，PFGE是调查猪痢疾暴发分子流行病学的有力技术。
