Pruckler J M, Masse N, Stevens V A, Gang L, Yang Y, Zell E R, Dowell S F, Fields B S
Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
J Clin Microbiol. 1999 Oct;37(10):3399-401. doi: 10.1128/JCM.37.10.3399-3401.1999.
Three methods for the recovery of Chlamydia pneumoniae from spiked nasopharyngeal and blood specimens, including extended culture and additional centrifugations, were compared. Additional centrifugations and a 7-day culture time resulted in a 500- to 5, 000-fold increase in the number of detectable inclusion-forming units.
对三种从加标的鼻咽和血液标本中回收肺炎衣原体的方法进行了比较,包括延长培养时间和增加离心次数。增加离心次数并延长培养7天,可使可检测到的包涵体形成单位数量增加500至5000倍。