一种通过胰蛋白酶或乙二胺四乙酸（EDTA）处理来分离肺炎衣原体的新方法。
A novel method for isolation of Chlamydia pneumoniae by treatment with trypsin or EDTA.
作者信息
Kazuyama Y, Lee S M, Amamiya K, Taguchi F
机构信息
Research and Development Center of Hygienic Science, Kitasato University, Minatoku, Tokyo, Japan.
出版信息
J Clin Microbiol. 1997 Jun;35(6):1624-6. doi: 10.1128/jcm.35.6.1624-1626.1997.
Abstract
To establish a novel method for the efficient isolation of Chlamydia pneumoniae, experiments were performed to determine the effects of EDTA or trypsin treatment of C. pneumoniae on its adsorption and inclusion body formation. Treatment of C. pneumoniae with 0.1% trypsin or 1 mM EDTA significantly increased inclusion body-forming activity from 8,000- to 10,000-fold higher than that of the control. C. pneumoniae was successfully isolated in cultured cells which were inoculated with clinical specimens after treatment with 0.1% trypsin.
摘要
为建立一种高效分离肺炎衣原体的新方法，进行了实验以确定用乙二胺四乙酸（EDTA）或胰蛋白酶处理肺炎衣原体对其吸附和包涵体形成的影响。用0.1%胰蛋白酶或1 mM EDTA处理肺炎衣原体后，包涵体形成活性显著提高，比对照高8000至10000倍。在用0.1%胰蛋白酶处理临床标本后接种的培养细胞中成功分离出肺炎衣原体。
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