使用多重聚合酶链反应检测葡萄球菌分离株中经典和新描述的致热毒素基因。
Use of multiplex PCR to detect classical and newly described pyrogenic toxin genes in staphylococcal isolates.
作者信息
Monday S R, Bohach G A
机构信息
Department of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, Idaho 83844, USA.
出版信息
J Clin Microbiol. 1999 Oct;37(10):3411-4. doi: 10.1128/JCM.37.10.3411-3414.1999.
Abstract
Staphylococcus aureus may contain one or more genes that encode a variety of immunomodulatory pyrogenic toxins (PTs), including the staphylococcal enterotoxins and toxic shock syndrome toxin (TSST). The PTs interact with several cellular targets to produce disease, such as food poisoning and toxic shock syndrome. At present, nine serologically distinct enterotoxins and one immunoreactive form of TSST have been identified and characterized. As isolates of S. aureus are further assessed, it is anticipated that this number will increase. To facilitate screening, a multiplex PCR was designed to simultaneously determine which of these 10 currently known PT genes an individual S. aureus isolate possesses. We show here, using S. aureus isolates with characterized PT phenotypes, that this novel PCR technique reliably detects each of the known PTs in a single reaction.
摘要
金黄色葡萄球菌可能含有一个或多个编码多种免疫调节性致热毒素（PTs）的基因，包括葡萄球菌肠毒素和中毒性休克综合征毒素（TSST）。这些PTs与多种细胞靶点相互作用从而引发疾病，如食物中毒和中毒性休克综合征。目前，已鉴定并表征了九种血清学上不同的肠毒素和一种TSST的免疫反应形式。随着对金黄色葡萄球菌分离株的进一步评估，预计这一数量将会增加。为便于筛查，设计了一种多重PCR，以同时确定单个金黄色葡萄球菌分离株拥有这10种目前已知的PT基因中的哪些。我们在此表明，使用具有已表征PT表型的金黄色葡萄球菌分离株，这种新型PCR技术能够在单一反应中可靠地检测出每种已知的PT。
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