Applied Microbial Processes & Environmental Health Research Group, Faculty of Life Sciences, University of Benin, PMB 1154, Benin City, 300283, Nigeria.
Department of Microbiology, College of Natural and Applied Sciences, Western Delta University, Oghara, Nigeria.
Sci Rep. 2024 Jun 9;14(1):13235. doi: 10.1038/s41598-024-63963-3.
The study investigated the economic concerns associated with livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in livestock (cow), examining its connection to severe infections, antimicrobial resistance (AMR), and virulence factors. The research, conducted in Edo State, Nigeria, analyzed 400 samples (200 rectal and 200 nasal swabs) collected between March 2018 and February 2019. MRSA prevalence was identified using conventional culture-based methods and polymerase chain reaction (PCR) techniques, revealing 63.5% (n = 254) for Staphylococcus aureus and 55% (n = 220) for MRSA. Of the 76 mecA-positive MRSA isolates, 64.5% (n = 49) exhibited multidrug resistance (MDR) while the remaining were sensitive to specific antimicrobials. Key virulence genes, such as PVL (81.6%; n = 62) and tsst-1 (44.7%; n = 34), were prevalent, along with AMR genes like mecC, tetM, ermA, ermC, vanA, and vanC. Staphylococcal chromosomal cassette mec (SCCmec) typing identified different types, notably II, IVa, and IVb. Biofilm formation, a crucial virulence factor varied in strength, is associated with icaA and icaB genes (p < 0.01). The findings highlighted substantial AMR and biofilm-forming capacity within LA-MRSA isolates, emphasizing the importance of ongoing surveillance for informed treatment strategies, AMR policies, and control measures against MDR staphylococcal infections.
本研究调查了与牲畜相关的耐甲氧西林金黄色葡萄球菌（LA-MRSA）在牲畜（奶牛）中引起的经济问题，研究了其与严重感染、抗微生物药物耐药性（AMR）和毒力因子的关系。该研究在尼日利亚埃多州进行，分析了 2018 年 3 月至 2019 年 2 月期间采集的 400 个样本（200 个直肠和 200 个鼻腔拭子）。使用传统的基于培养的方法和聚合酶链反应（PCR）技术确定 MRSA 的流行率，结果显示金黄色葡萄球菌的流行率为 63.5%（n=254），耐甲氧西林金黄色葡萄球菌的流行率为 55%（n=220）。在 76 株 mecA 阳性耐甲氧西林金黄色葡萄球菌分离株中，64.5%（n=49）表现出多药耐药性（MDR），其余对特定抗菌药物敏感。流行的关键毒力基因包括 PVL（81.6%；n=62）和 tsst-1（44.7%；n=34），以及 AMR 基因，如 mecC、tetM、ermA、ermC、vanA 和 vanC。葡萄球菌染色体盒 mec（SCCmec）分型确定了不同类型，主要是 II、IVa 和 IVb。生物膜形成是一种重要的毒力因子，其强度因 icaA 和 icaB 基因而异（p<0.01）。研究结果强调了 LA-MRSA 分离株中存在的大量 AMR 和生物膜形成能力，这表明需要持续监测，以制定明智的治疗策略、AMR 政策和控制多药耐药性葡萄球菌感染的措施。
