Fevereiro M, Barros S, Fagulha T
Laboratório Nacional de Investigação Veterinária, Dept. Virologia, Lisbon, Portugal.
J Virol Methods. 1999 Aug;81(1-2):101-8. doi: 10.1016/s0166-0934(99)00061-0.
A monoclonal antibody (MAb) blocking ELISA (Blck-ELISA) was developed to detect antibodies against Maedi-Visna virus (MVV) in sheep sera. The assay employs a MAb directed against the envelope protein p90 of the virus in a sandwich blocking procedure. To determine whether the MAb was a potential antibody for developing a Blck-ELISA, a collection of three hundred sera obtained from several sheep flocks known to be infected with MVV were used to examine the sensitivity of the Blck-ELISA. A total of 50 serum samples originating from a flock free of MVV were tested to assess the specificity of the assay. The results were compared with a commercial indirect ELISA (I-ELISA) and samples giving a conflicting or doubtful result were tested by immunoblot. The Blck-ELISA proved to be specific, sensitive and it showed high reproducibility and low variability.
开发了一种单克隆抗体（MAb）阻断酶联免疫吸附测定法（Blck-ELISA），用于检测绵羊血清中抗梅迪-维斯纳病毒（MVV）的抗体。该测定法在夹心阻断程序中采用针对该病毒包膜蛋白p90的单克隆抗体。为了确定该单克隆抗体是否是开发Blck-ELISA的潜在抗体，使用从已知感染MVV的几个羊群中获得的三百份血清样本，检测Blck-ELISA的灵敏度。总共检测了来自无MVV羊群的50份血清样本，以评估该测定法的特异性。将结果与商业间接酶联免疫吸附测定法（I-ELISA）进行比较，并通过免疫印迹法检测给出相互矛盾或可疑结果的样本。结果表明，Blck-ELISA具有特异性、敏感性，且重现性高、变异性低。
