Simard C L, Briscoe M R
Food Production and Inspection Branch, Agriculture Canada, Sackville, New Brunswick.
Can J Vet Res. 1990 Oct;54(4):451-6.
A study was conducted to compare the indirect enzyme-linked immunosorbent-assay (i-ELISA) test using antigen prepared by a simple technique using sodium dodecyl sulfate (SDS) treatment to the conventional agar gel immunodiffusion test (AGID). Ten specific-pathogen-free (SPF) sheep were inoculated with maedi-visna virus (MVV) and serum antibody titers compared over a period of 14 weeks. All the sheep seroconverted by the i-ELISA compared to 90% by the AGID. The i-ELISA detected antibody at a mean of 2.6 weeks prior to the AGID. In both tests, fluctuations were observed in the serum antibody response of two sheep. The i-ELISA had a specificity of at least 98.8% and an increased relative sensitivity of 15.5% compared to the AGID, based on the analysis of sera from experimental sheep with MVV free status and sera from sheep from various sources. Of the sera from a seronegative flock which had been monitored with the AGID after a "test and remove" eradication program, 10.2% were positive by the i-ELISA. It was concluded that the AGID test may not be adequate to monitor samples for an eradication scheme.
开展了一项研究，以比较使用经十二烷基硫酸钠（SDS）处理的简单技术制备的抗原进行间接酶联免疫吸附测定（i-ELISA）试验与传统琼脂凝胶免疫扩散试验（AGID）。给10只无特定病原体（SPF）绵羊接种梅迪 - 维斯纳病毒（MVV），并在14周内比较血清抗体滴度。与AGID检测的90%相比，所有绵羊通过i-ELISA检测均发生血清转化。i-ELISA比AGID平均提前2.6周检测到抗体。在两项试验中，均观察到两只绵羊的血清抗体反应存在波动。基于对无MVV状态的实验绵羊血清以及来自不同来源绵羊血清的分析，i-ELISA的特异性至少为98.8%，相对灵敏度比AGID提高了15.5%。在一个经过“检测并清除”根除计划后用AGID监测的血清阴性羊群的血清中，10.2%通过i-ELISA检测呈阳性。得出的结论是，AGID试验可能不足以监测用于根除计划的样本。
