Dubey J P, Venturini M C, Venturini L, McKinney J, Pecoraro M
Parasite Biology and Epidemiology Laboratory, United States Department of Agriculture, Agricultural Research Service, Livestock and Poultry Sciences Institute, Beltsville, MD 20705-2350, USA.
Vet Parasitol. 1999 Sep 15;86(1):59-62. doi: 10.1016/s0304-4017(99)00127-2.
Sera from 76 horses from Argentina were examined for antibodies to Sarcocystis neurona, Toxoplasma gondii and Neospora caninum. Antibodies to S. neurona were found in 27 (35.5%) of 76 horses using immunoblots with culture derived merozoites as antigen. Antibodies to T. gondii were found in 10 (13.1%) of 76 horses by using the modified agglutination test with formalin-fixed tachyzoites and mercaptoethanol; titers were 1:25 (two horses), 1:50 (six horses), 1:100 (two horses), and 1:200 (one horse). Antibodies to N. caninum were not found in any of the 76 horses by the use of N. caninum agglutination test. This is the first report of S. neurona infection in horses in Argentina.
对来自阿根廷的76匹马的血清进行了检测,以查找抗马神经肉孢子虫、刚地弓形虫和犬新孢子虫的抗体。使用源自培养物的裂殖子作为抗原进行免疫印迹法检测,在76匹马中有27匹(35.5%)检测到抗马神经肉孢子虫抗体。通过使用甲醛固定速殖子和巯基乙醇的改良凝集试验,在76匹马中有10匹(13.1%)检测到抗刚地弓形虫抗体;滴度分别为1:25(两匹马)、1:50(六匹马)、1:100(两匹马)和1:200(一匹马)。通过犬新孢子虫凝集试验,在76匹马中均未检测到抗犬新孢子虫抗体。这是阿根廷马匹感染马神经肉孢子虫的首次报告。