接受生物人工肝支持系统治疗的患者未感染猪内源性逆转录病毒（PERV）的证据。

Evidence of absence of porcine endogenous retrovirus (PERV) infection in patients treated with a bioartificial liver support system.

作者信息

Pitkin Z, Mullon C

机构信息

Circe Biomedical, Incorporated, Lexington, Massachusetts 02421, USA.

出版信息

Artif Organs. 1999 Sep;23(9):829-33. doi: 10.1046/j.1525-1594.1999.06444.x.

DOI:10.1046/j.1525-1594.1999.06444.x

PMID:10491030

Abstract

Porcine endogenous retrovirus (PERV) genomes are present in all pig cells. In this retrospective study, we assessed PERV infectivity in 28 patients treated with an extracorporeal bioartificial liver (HepatAssist system) that includes a membrane device containing porcine hepatocytes. All patients tested negative for PERV using polymerase chain reaction analysis of peripheral blood mononuclear cells (PBMC) collected up to 5 years after treatment. In vitro results showed that the membrane decreased the risk of PERV transmission by a factor of 105, and porcine hepatocytes did not produce infectious PERV in co-cultures with human cell line 293. Our results do not support the presence of PERV infection in patients treated with this porcine hepatocyte-based bioartificial liver.

摘要

猪内源性逆转录病毒（PERV）基因组存在于所有猪细胞中。在这项回顾性研究中，我们评估了28例接受体外生物人工肝（HepatAssist系统）治疗的患者的PERV感染性，该系统包括一个含有猪肝细胞的膜装置。使用聚合酶链反应分析治疗后长达5年收集的外周血单个核细胞（PBMC），所有患者的PERV检测均为阴性。体外结果表明，该膜将PERV传播风险降低了105倍，并且猪肝细胞与人细胞系293共培养时未产生具有感染性的PERV。我们的结果不支持接受这种基于猪肝细胞的生物人工肝治疗的患者存在PERV感染。