Lorenzen E, Carstensen B, Olesen N J
Danish Veterinary Laboratory, EU Reference Laboratory for Fish Diseases, Arhus, Denmark.
Dis Aquat Organ. 1999 Jul 30;37(2):81-8. doi: 10.3354/dao037081.
Eleven European National Reference Laboratories participated in an inter-laboratory comparison of the susceptibility of 5 selected cell lines to 3 fish pathogenic viruses. The test included viral hemorrhagic septicaemia virus (VHSV); infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV), and the cell lines derived from bluegill fry (BF-2), chinook salmon embryo (CHSE-214), epithelioma papulosum cyprini (EPC), fathead minnow (FHM) and rainbow trout gonad (RTG-2). The results showed that for isolation of VHSV, BF-2 and RTG-2 cells performed equally well and had higher sensitivity compared to the other cell lines. For IHNV, EPC and FHM cells gave the best results, and for IPNV it was BF-2 and CHSE-214 cells. FHM cells showed the largest variability among laboratories, whereas EPC was the cell line showing the smallest variability.
11个欧洲国家参考实验室参与了一项关于5种选定细胞系对3种鱼类致病病毒易感性的实验室间比对。该测试包括病毒性出血性败血症病毒（VHSV）、传染性造血器官坏死病毒（IHNV）和传染性胰脏坏死病毒（IPNV），以及来自蓝鳃太阳鱼幼体（BF - 2）、奇努克鲑胚胎（CHSE - 214）、鲤上皮瘤（EPC）、黑头软口鲦（FHM）和虹鳟性腺（RTG - 2）的细胞系。结果表明，对于VHSV的分离，BF - 2和RTG - 2细胞表现同样出色，且与其他细胞系相比具有更高的敏感性。对于IHNV，EPC和FHM细胞给出了最佳结果，而对于IPNV，则是BF - 2和CHSE - 214细胞。FHM细胞在各实验室间表现出的变异性最大，而EPC是变异性最小的细胞系。
