Silveira T G, Arraes S M, Bertolini D A, Teodoro U, Lonardoni M V, Roberto A C, Ramos M, Nerilo Sobrinho A, Ishikawa E, Shaw J
Departamento de Análises Clínicas da Universidade Estadual de Maringá, PR, Brasil.
Rev Soc Bras Med Trop. 1999 Jul-Aug;32(4):413-23. doi: 10.1590/s0037-86821999000400013.
Between 1986 and 1997 a total of 1418 patients were examined at the Clinical Analysis Teaching and Research Laboratory of Maringá State University (LEPAC/UEM) for cutaneous leishmaniasis by direct examination of stained smears made from the lesions, the Montenegro skin test and the indirect immunofluorescent antibody test. Nine hundred and fifty five patients (67.3%) were positive for at least one of the three tests and of these 804 (84.2%) were considered to have contracted the disease in Paraná State; 665 (69.6%) were between 15 and 49 years old; 658 (68.9%) were males; 523 (54.8%) sought medical advice during the first three months of their infections and 74 (7.7%) had mucosal lesions. Of the 83 counties of Paraná State, where the patients had most probably acquired their infections, 44.7% were from the counties of São Jorge do Ivaí (10.2%), Doutor Camargo (9.8%), Terra Boa (7.3%), Maringá (7.3%), Jussara (6.0%) and Cianorte (4.5%). Seventy seven strains of Leishmania (Viannia) braziliensis were isolated and 63.6% of these strains belong to serodema 1.
1986年至1997年间,马林加州立大学临床分析教学与研究实验室(LEPAC/UEM)对1418名患者进行了皮肤利什曼病检查,检查方法包括对病变部位制作的染色涂片进行直接检查、 Montenegro皮肤试验和间接免疫荧光抗体试验。955名患者(67.3%)至少一项检查呈阳性,其中804名(84.2%)被认为是在巴拉那州感染该病;665名(69.6%)年龄在15至49岁之间;658名(68.9%)为男性;523名(54.8%)在感染后的前三个月寻求医疗建议,74名(7.7%)有黏膜病变。在患者最有可能感染的巴拉那州的83个县中,44.7%来自圣若热-杜伊瓦伊县(10.2%)、卡马古医生县(9.8%)特鲁阿县(7.3%)、马林加县(7.3%)、朱萨拉县(6.0%)和西亚诺尔蒂县(4.5%)共分离出77株巴西利什曼原虫(维阿尼亚种),其中63.6%的菌株属于血清型1。