Zavala-Velazquez J E, Ruiz-Sosa J, Vado-Solis I, Billings A N, Walker D H
Facultad de Medicina, Universidad Autonoma de Yucatán, Merida, Mexico.
Am J Trop Med Hyg. 1999 Sep;61(3):405-8. doi: 10.4269/ajtmh.1999.61.405.
Because of the discovery of a spotted fever group rickettsiosis with signs and symptoms similar to dengue fever in Yucatan, Mexico, immunofluorescence assay (IFA) serology was performed on sera from 390 persons selected from a representative geographic distribution of rural Yucatan to detect antibodies reactive with Rickettsia rickettsii, R. akari, a Thai strain (TT-118) that is most closely related to a rickettsia identified in Amblyomma cajennense ticks in southern Texas, and R. typhi. The IFA antibodies at titers > or = 1:64 against R. akari were detected in 22 (5.6%) of the samples with the expected cross-reactivity against the other antigens of the spotted fever group. Immunoblotting with antigens of R. akari identified antibodies against antigens of spotted fever group lipopolysaccharides and not against rickettsial outer membrane proteins A and B, which contain the species-specific epitopes. A rickettsiosis most likely caused by a relative of R. akari appears to be both prevalent and widely distributed geographically in Yucatan.
由于在墨西哥尤卡坦发现了一种斑点热群立克次体病，其体征和症状与登革热相似，因此对从尤卡坦农村具有代表性地理分布中选取的390人的血清进行了免疫荧光测定（IFA）血清学检测，以检测与立氏立克次体、小蛛立克次体、一种与在得克萨斯州南部卡延钝眼蜱中鉴定出的立克次体关系最密切的泰国菌株（TT - 118）以及伤寒立克次体发生反应的抗体。在22份（5.6%）样本中检测到针对小蛛立克次体的IFA抗体效价≥1:64，并且对斑点热群的其他抗原具有预期的交叉反应性。用小蛛立克次体抗原进行免疫印迹分析，鉴定出针对斑点热群脂多糖抗原的抗体，而不是针对含有种特异性表位的立克次体外膜蛋白A和B的抗体。一种很可能由小蛛立克次体的亲缘种引起的立克次体病在尤卡坦似乎既普遍又广泛分布于地理区域。
