固定化单光捕获复合体的结构变形动力学
The dynamics of structural deformations of immobilized single light-harvesting complexes.
作者信息
Bopp M A, Sytnik A, Howard T D, Cogdell R J, Hochstrasser R M
机构信息
Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA.
出版信息
Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11271-6. doi: 10.1073/pnas.96.20.11271.
Abstract
Single assemblies of the intact light-harvesting complex LH2 from Rhodopseudomonas acidophila were bound to mica surfaces at 300 K and examined by observing their fluorescence after polarized light excitation. The complexes are generally not cylindrically symmetric. They act like elliptic absorbers, indicating that the high symmetry found in crystals of LH2 is not present when the molecules are immobilized on mica. The ellipticity and the principal axes of the ellipses fluctuate on the time scale of seconds, indicating that there is a mobile structural deformation. The B850 ring of cofactors shows significantly less asymmetry than B800. The photobleaching strongly depends on the presence of oxygen.
摘要
来自嗜酸红假单胞菌的完整捕光复合物LH2的单个聚集体在300 K下结合到云母表面，并通过观察其在偏振光激发后的荧光进行检测。这些复合物通常不是圆柱对称的。它们的作用类似于椭圆吸收体，这表明当分子固定在云母上时，LH2晶体中发现的高对称性不存在。椭圆率和椭圆的主轴在秒的时间尺度上波动，表明存在可移动的结构变形。辅因子的B850环显示出的不对称性明显小于B800。光漂白强烈依赖于氧气的存在。
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