Hanania U, Furman-Matarasso N, Ron M, Avni A
Department of Plant Sciences, Tel Aviv University, Israel.
Plant J. 1999 Sep;19(5):533-41. doi: 10.1046/j.1365-313x.1999.00547.x.
Challenging tomato or tobacco varieties with ethylene-inducing xylanase (EIX) from the fungus Trichoderma viride causes rapid induction of plant defence responses leading to programmed cell death. Using the yeast two-hybrid system, we isolated a novel protein, tomato small ubiquitin-related modifier protein (T-SUMO), which specifically interacts with EIX. T-SUMO, a cytoplasmic protein, is a member of the ubiquitin-like protein family. It shows homology to human protein sentrin/SUMO1, which suppresses tumour necrosis factor-induced cell death. Transgenic plants that express T-SUMO in the sense orientation suppress EIX induction of ethylene biosynthesis and cell death, while in the antisense orientation they enhance EIX-induced ethylene biosynthesis. These results indicate that T-SUMO is involved in mediating the signal generated by EIX that leads to induction of plant defence responses.
用来自绿色木霉的乙烯诱导木聚糖酶（EIX）处理番茄或烟草的挑战性品种，会迅速诱导植物防御反应，导致程序性细胞死亡。利用酵母双杂交系统，我们分离出一种新型蛋白质，番茄小泛素相关修饰蛋白（T-SUMO），它与EIX特异性相互作用。T-SUMO是一种细胞质蛋白，属于泛素样蛋白家族成员。它与人源蛋白sentrin/SUMO1具有同源性，后者可抑制肿瘤坏死因子诱导的细胞死亡。以正义方向表达T-SUMO的转基因植物抑制EIX诱导的乙烯生物合成和细胞死亡，而以反义方向表达时则增强EIX诱导的乙烯生物合成。这些结果表明，T-SUMO参与介导由EIX产生的导致植物防御反应诱导的信号。
