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采用双重梯度-变性梯度凝胶电泳法检测结核分枝杆菌中的利福平耐药性。

Detection of rifampin resistance in Mycobacterium tuberculosis by double gradient-denaturing gradient gel electrophoresis.

作者信息

Scarpellini P, Braglia S, Carrera P, Cedri M, Cichero P, Colombo A, Crucianelli R, Gori A, Ferrari M, Lazzarin A

机构信息

Infectious Diseases Division, San Raffaele Scientific Institute, University of Milan, Milan, Italy.

出版信息

Antimicrob Agents Chemother. 1999 Oct;43(10):2550-4. doi: 10.1128/AAC.43.10.2550.

DOI:10.1128/AAC.43.10.2550
PMID:10508043
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC89519/
Abstract

We applied double gradient-denaturing gradient gel electrophoresis (DG-DGGE) for the rapid detection of rifampin (RMP) resistance from rpoB PCR products of Mycobacterium tuberculosis isolates and clinical samples. The results of this method were fully concordant with those of DNA sequencing and susceptibility testing analyses. DG-DGGE is a valid alternative to the other methods of detecting mutations for predicting RMP resistance.

摘要

我们应用双梯度变性梯度凝胶电泳（DG-DGGE）从结核分枝杆菌分离株和临床样本的rpoB聚合酶链反应（PCR）产物中快速检测利福平（RMP）耐药性。该方法的结果与DNA测序和药敏试验分析的结果完全一致。DG-DGGE是预测RMP耐药性的其他检测突变方法的有效替代方法。

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