Tang Peijun, Wang Xiafang, Shen Xinghua, Shi Meihua, Zhu Xuefeng, Yu Xin, Liu Jia, Ling Chunhua, Wu Meiying
Department of Respiration, The First Hospital Affiliated to Soochow University, Suzhou, Jiangsu 215007, P.R. China.
Department of Tuberculosis, The Affiliated Infectious Hospital of Soochow University, The Fifth People's Hospital of Suzhou, Suzhou, Jiangsu 215007, P.R. China.
Exp Ther Med. 2017 May;13(5):2332-2338. doi: 10.3892/etm.2017.4250. Epub 2017 Mar 21.
The objective of the present study was to evaluate the potential development of DNA microarray chips to detect rifampicin (RFP) and isoniazid (INH) resistance in (MTB), using samples from clinical tuberculosis (TB) patients in Soochow City, China. The sputum samples of 42 patients with TB in the Affiliated Hospital of Infectious Diseases of Soochow University (Soochow, China) were collected. The conventional Lowenstein-Jensen culture medium (Gold Standard) was used to assess drug sensitivity using the absolute concentration method. GeeDom MTB drug detection kits were also used to create a DNA microarray chip and examine the RFP-resistance associated gene mutation points and , and the INH-resistance associated gene mutation points and of the sputum samples. Compared with the results from the absolute concentration method, the susceptibility and specificity of RFP sensitivity detected by the DNA microarray chip were 92.8 and 93.8%, respectively. The susceptibility and specificity of INH sensitivity detected were 66.7 and 81%, respectively. The mutations were the primary causes of MTB RFP resistance and the mutation was the primary cause of INH resistance. The detection of and gene mutation points by a DNA microarray chip may be used as a rapid, accurate and bulk clinical detection method for RFP and INH resistance in MTB. This is very valuable for the control of TB epidemics.
本研究的目的是利用中国苏州市临床结核病患者的样本，评估DNA微阵列芯片检测结核分枝杆菌（MTB）对利福平（RFP）和异烟肼（INH）耐药性的潜在发展情况。收集了苏州大学附属传染病医院（中国苏州）42例结核病患者的痰液样本。使用传统的罗氏培养基（金标准），采用绝对浓度法评估药物敏感性。还使用基迪奥MTB药物检测试剂盒制作DNA微阵列芯片，并检测痰液样本中与RFP耐药相关的基因突变点rpoB和katG，以及与INH耐药相关的基因突变点inhA和kasA。与绝对浓度法的结果相比，DNA微阵列芯片检测RFP敏感性的敏感性和特异性分别为92.8%和93.8%。检测INH敏感性的敏感性和特异性分别为66.7%和81%。rpoB突变是MTB对RFP耐药的主要原因，katG突变是INH耐药的主要原因。通过DNA微阵列芯片检测rpoB和katG基因突变点，可作为一种快速、准确、批量检测MTB对RFP和INH耐药性的临床检测方法。这对控制结核病流行非常有价值。
