Gustafsson M G
Department of Biochemistry, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143-0448, USA.
Curr Opin Struct Biol. 1999 Oct;9(5):627-34. doi: 10.1016/s0959-440x(99)00016-0.
Fluorescence microscopy is an essential tool of modern biology, but, like all forms of optical imaging, it is subject to physical limits on its resolving power. In recent years, several exciting techniques have been introduced to exceed these limits, including standing wave microscopy, 4Pi confocal microscopy, I5M and structured illumination microscopy. Several such techniques have been definitively demonstrated for the first time during the past year.
荧光显微镜是现代生物学的一项重要工具，但与所有光学成像形式一样，其分辨率受到物理限制。近年来，已经引入了几种令人兴奋的技术来突破这些限制，包括驻波显微镜、4Pi共聚焦显微镜、I5M和结构照明显微镜。在过去一年中，首次明确展示了几种此类技术。
