Latour X, Philippot L, Corberand T, Lemanceau P
Laboratoire de Recherches sur la Flore Pathogène dans le Sol, INRA-CMSE, 17 rue Sully, 21034, Dijon, Cedex, France
FEMS Microbiol Ecol. 1999 Oct 1;30(2):163-170. doi: 10.1111/j.1574-6941.1999.tb00645.x.
Indigenous populations of fluorescent pseudomonads were previously shown to vary in two different soils (Châteaurenard and Dijon) and in the rhizosphere of a plant species (Linum usitatissimum L.) cultivated in these two soils. These differences could be related to the soil type and to their crop history. In the present study, the influence of the soil type on the diversity of fluorescent pseudomonads in bulk and rhizospheric soils was evaluated. The soils of Châteaurenard and Dijon were sterilized before being inoculated with the same community of fluorescent pseudomonads. Bacterial isolates from bulk and rhizospheric soils were characterized on the basis of their repetitive extragenic palindromic-PCR patterns allowing the comparison of the distributions of the introduced populations. The influence of the soil type on the establishment of the introduced bacterial community was recorded in the two bulk soils. Indeed, the density and the structure of the community differed significantly between the two soils. The rhizosphere effect was also affected by the soil type. This effect was expressed in the Châteaurenard soil but not in the Dijon soil. Hence, the structure of the bacterial community associated with the roots of the same plant species cultivated in the two soils differed significantly.
先前的研究表明，荧光假单胞菌的本地种群在两种不同的土壤（沙托雷诺和第戎）以及在这两种土壤中种植的一种植物（亚麻）的根际中存在差异。这些差异可能与土壤类型及其种植历史有关。在本研究中，评估了土壤类型对土壤本体和根际土壤中荧光假单胞菌多样性的影响。在接种相同的荧光假单胞菌群落之前，将沙托雷诺和第戎的土壤进行了灭菌处理。根据细菌的重复外显子回文-PCR模式对土壤本体和根际土壤中的细菌分离株进行了表征，从而可以比较引入种群的分布情况。在两种土壤本体中记录了土壤类型对引入细菌群落定殖的影响。实际上，两种土壤中群落的密度和结构存在显著差异。根际效应也受土壤类型的影响。这种效应在沙托雷诺土壤中表现出来，但在第戎土壤中未表现出来。因此，在两种土壤中种植的同一植物物种根系相关细菌群落的结构存在显著差异。
