与根相关的荧光假单胞菌种群的组成受植物和土壤类型的影响。
The composition of fluorescent pseudomonad populations associated with roots is influenced by plant and soil type.
出版信息
Appl Environ Microbiol. 1996 Jul;62(7):2449-56. doi: 10.1128/aem.62.7.2449-2456.1996.
Populations of fluorescent pseudomonads isolated from an uncultivated soil and from the roots of two plant species were previously shown to differ (P. Lemanceau, T. Corberand, L. Gardan, X. Latour, G. Laguerre, J.-M. Boeufgras, and C. Alabouvette, Appl. Environ. Microbiol. 61:1004-1012, 1995). The diversities of fluorescent pseudomonads, from two uncultivated soils and from the roots of two plant species cultivated in these two soils, were compared. The phenotypic diversity of the bacterial isolates was characterized on the basis of biochemical and physiological tests and on the basis of their ability to utilize 147 different organic compounds. The genotypic diversity of the isolates was characterized on the basis of the types of 16S genes coding for rRNA (rDNA), their repetitive extragenic palindromic patterns by PCR, and plasmid profiles. Taxonomic identification of the isolates was achieved with both biochemical and physiological tests and by comparing their 16S rDNA types to those of reference and type strains of fluorescent Pseudomonas spp. Numerical analysis of phenotypic characteristics allowed the clustering of isolates that showed high levels of similarity. This analysis indicated that both soil type and host plant had an effect on the diversity of fluorescent pseudomonads. However, of the two factors studied, the soil was clearly the dominating one. Indeed, the populations associated with the roots of each plant species varied from one soil to the other. This variation could possibly be ascribed to the differences recorded between the phenotypically diverse populations of fluorescent pseudomonads from the two uncultivated soils. The plant selection was, at least partly, plant specific. It was not related to bacterial species and biovars or to the presence of plasmid DNA. The phenotypic clustering of isolates was well correlated with genotypic characterization by repetitive extragenic palindrome-PCR fingerprinting.
先前已证明，从未培养的土壤和两种植物根部分离到的荧光假单胞菌种群存在差异（P. Lemanceau、T. Corberand、L. Gardan、X. Latour、G. Laguerre、J.-M. Boeufgras 和 C. Alabouvette，应用环境微生物学。61:1004-1012,1995）。比较了来自两种未培养土壤和在这两种土壤中种植的两种植物根部的荧光假单胞菌的多样性。细菌分离物的表型多样性是基于生化和生理测试以及它们利用 147 种不同有机化合物的能力来描述的。分离物的基因型多样性是基于编码 rRNA（rDNA）的 16S 基因类型、PCR 重复外回文模式和质粒图谱来描述的。通过生化和生理测试以及将其 16S rDNA 类型与荧光假单胞菌属的参考和模式菌株进行比较，对分离物进行了分类鉴定。表型特征的数值分析允许对表现出高度相似性的分离物进行聚类。该分析表明，土壤类型和宿主植物都会影响荧光假单胞菌的多样性。然而，在所研究的两个因素中，土壤显然是主导因素。实际上，与每种植物根部相关的种群在两种土壤之间存在差异。这种变化可能归因于从两种未培养土壤中分离出的荧光假单胞菌在表型上的差异。植物的选择至少部分是特定于植物的。它与细菌种类和生物型或质粒 DNA 的存在无关。分离物的表型聚类与重复外回文-PCR 指纹图谱的基因型特征很好地相关。
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