Barker M G, Walmsley R M
Department of Biomolecular Sciences, UMIST, PO Box 88, Manchester M60 1QD, UK.
Yeast. 1999 Oct;15(14):1511-8. doi: 10.1002/(sici)1097-0061(199910)15:14<1511::aid-yea482>3.3.co;2-p.
Saccharomyces cerevisiae has been widely used as a model organism in studies of replicative ageing and senescence. The relevance of these studies to ageing in other organisms has, however, been questioned, since this yeast divides by budding rather than fission, the more common pattern in higher organisms. Here we report that, contrary to popular belief, the fission yeast Schizosaccharomyces pombe also undergoes replicative senescence and in a manner superficially analogous to budding yeast. These experiments provide the first evidence of age asymmetry in cell fission and are consistent with the hypothesis of Jazwinski, that asymmetric division underlies culture immortality. Given their evolutionary divergence, comparison of the ageing determinants in fission and budding yeasts may help identify common mechanisms of the ageing process.
酿酒酵母已被广泛用作研究复制性衰老和衰老的模式生物。然而，这些研究与其他生物体衰老的相关性受到了质疑，因为这种酵母通过出芽而不是裂变进行分裂，而裂变是高等生物中更常见的模式。我们在此报告，与普遍看法相反，裂殖酵母粟酒裂殖酵母也会经历复制性衰老，并且其方式与出芽酵母表面上类似。这些实验提供了细胞分裂中年龄不对称的首个证据，并且与贾兹温斯基的假设一致，即不对称分裂是培养永生的基础。鉴于它们在进化上的差异，比较裂殖酵母和出芽酵母中的衰老决定因素可能有助于识别衰老过程的共同机制。
