Vian A, Henry-Vian C, Davies E
Department of Botany, Box 7612, North Carolina State University, Raleigh, North Carolina 27695-7612, USA.
Plant Physiol. 1999 Oct;121(2):517-24. doi: 10.1104/pp.121.2.517.
It has been shown that tomato (Lycopersicon esculentum) plants respond to flame wounding and electrical stimulation by a rapid (15 min) and systemic up-regulation of proteinase inhibitor (pin) genes. To find other genes having a similar expression pattern, we used subtractive cDNA screening between flamed and control plants to select clones up-regulated by flame wounding. We report the characterization of one of them, a chloroplast mRNA-binding protein encoded by a single gene and expressed preferentially in the leaves. Systemic gene expression in response to flaming in the youngest terminal leaf exhibited three distinct phases: a rapid and transient increase (5-15 min) in transcript accumulation, a decline to basal levels (15-45 min), and then a second, more prolonged increase (60-90 min). In contrast, after a mechanical wound the rapid, transient increase (5 min) was followed by a rapid decline to basal levels but no later, prolonged accumulation. In the petiole, the initial flame-wound-evoked transient increase (15 min) was followed by a continuous decline for 3 h. The nature of the wound signal(s) causing such rapid changes in transcript abundance is discussed in relation to electrical signaling, which has recently been implicated in plant responses to wounding.
研究表明，番茄（Lycopersicon esculentum）植株对火焰伤害和电刺激的反应是，蛋白酶抑制剂（pin）基因迅速（15分钟）且系统性地上调。为了找到其他具有类似表达模式的基因，我们利用火焰处理植株和对照植株之间的消减cDNA筛选，来挑选因火焰伤害而上调的克隆。我们报道了其中一个基因的特征，它是一种由单基因编码的叶绿体mRNA结合蛋白，优先在叶片中表达。最幼嫩的顶叶对火焰处理的系统性基因表达呈现出三个不同阶段：转录本积累迅速且短暂增加（5 - 15分钟），下降至基础水平（15 - 45分钟），然后是第二次更持久的增加（60 - 90分钟）。相比之下，机械损伤后，迅速且短暂的增加（5分钟）之后是迅速下降至基础水平，但没有随后的持久积累。在叶柄中，最初由火焰伤害引起的短暂增加（15分钟）之后是持续3小时的下降。结合最近与植物对伤害反应相关的电信号传导，讨论了导致转录本丰度如此快速变化的伤口信号的性质。
