Pastuglia M, Roby D, Dumas C, Cock J M
Reconnaissance Cellulaire et Amélioration des Plantes, UMR 9938 CNCRS-INRA-ENSL, Ecole Normale Supérieure de Lyon, France.
Plant Cell. 1997 Jan;9(1):49-60. doi: 10.1105/tpc.9.1.49.
A receptor-like kinase, SRK, has been implicated in the autoincompatible response that leads to the rejection of self-pollen in Brassica plants. SRK is encoded by one member of a multigene family, which includes several receptor-like kinase genes with patterns of expression very different from that of SRK but of unknown function. Here, we report the characterization of a novel member of the Brassica S gene family, SFR2. RNA gel blot analysis demonstrated that SFR2 mRNA accumulated rapidly in response both to wounding and to infiltration with either of two bacteria: Xanthomonas campestris, a pathogen, and Escherichia coli, a saprophyte. SFR2 mRNA also accumulated rapidly after treatment with salicylic acid, a molecule that has been implicated in plant defense response signaling pathways. A SFR2 promoter and reporter gene fusion was introduced into tobacco and was shown to be induced by bacteria of another genus, Ralstonia (Pseudomonas) solanacearum. The accumulation of SFR2 mRNA in response to wounding and pathogen invasion is typical of a gene involved in the defense responses of the plant. The rapidity of SFR2 mRNA accumulation is consistent with SFR2 playing a role in the signal transduction pathway that leads to induction of plant defense proteins, such as pathogenesis-related proteins or enzymes of phenylpropanoid metabolism.
一种类受体激酶SRK与自交不亲和反应有关，该反应导致十字花科植物排斥自身花粉。SRK由一个多基因家族的成员编码，该家族包括几个类受体激酶基因，其表达模式与SRK非常不同，但功能未知。在此，我们报道了十字花科S基因家族一个新成员SFR2的特性。RNA凝胶印迹分析表明，SFR2 mRNA在受到创伤以及被两种细菌（致病的野油菜黄单胞菌和腐生的大肠杆菌）中的任何一种侵染后都会迅速积累。用已参与植物防御反应信号通路的水杨酸处理后，SFR2 mRNA也会迅速积累。将一个SFR2启动子与报告基因的融合体导入烟草，结果表明它能被另一个属的细菌——茄科雷尔氏菌（假单胞菌）诱导。SFR2 mRNA在响应创伤和病原体入侵时的积累是参与植物防御反应的基因的典型特征。SFR2 mRNA积累的快速性与SFR2在导致植物防御蛋白（如病程相关蛋白或苯丙烷代谢酶）诱导的信号转导途径中发挥作用相一致。
