极早产儿出生的学龄儿童外周血淋巴细胞亚群
Peripheral blood lymphocyte subpopulations in schoolchildren born very preterm.
作者信息
Pelkonen A S, Suomalainen H, Hallman M, Turpeinen M
机构信息
Department of Allergic Diseases, Helsinki University Central Hospital, Meilahdentie 2, POB 160 00029 Huch, Finland.
出版信息
Arch Dis Child Fetal Neonatal Ed. 1999 Nov;81(3):F188-93. doi: 10.1136/fn.81.3.f188.
AIM
To investigate whether lymphocytes or serum inflammatory markers are associated with obstructive lung disease and bronchial lability in schoolchildren born very preterm.
METHOD
Lymphocyte subsets were studied in the peripheral venous blood of 29 such children (median age 8.8 years). Serum eosinophil cationic protein (ECP) and myeloperoxidase (MPO) concentrations and the association between them, lymphocyte subsets, and lung function were studied. Fourteen healthy children born at term, median age 9.1 years, served as controls. T lymphocytes (CD3), T lymphocyte subpopulations (CD4 and CD8), B lymphocytes (CD19), natural killer cells (CD16+56) and activation markers of T and B lymphocytes (CD23 and CD25) were determined using flow cytometry. Lung function was measured in all children both in the clinic and at home (Vitalograph Data Storage Spirometer).
RESULTS
Compared with the controls, schoolchildren born very preterm had significantly lower CD4(+) T cell percentages and CD4:CD8 ratios (p < 0.05 for both), whereas natural killer cell percentages and serum ECP values were significantly higher (p < 0. 05). The very preterm schoolchildren had significantly lower spirometric values than the control group (p < 0.05)-except forced vital capacity. When all the subjects were considered together, a weak, but significant, negative association was observed between the bronchial responsiveness in peak expiratory flow, after a beta(2) agonist during home monitoring, and the CD4(+) T cell percentage (r = -0.45; p = 0.008) and the CD4:CD8 ratio (r = -0.50; p = 0.003), indicating a relation between bronchial lability and imbalance of T cell subpopulations.
CONCLUSIONS
These results suggest that there is an inflammatory basis for lung function abnormalities in schoolchildren born very preterm.
目的
研究淋巴细胞或血清炎症标志物是否与极早产出生的学龄儿童的阻塞性肺病及支气管易激性相关。
方法
对29名此类儿童（中位年龄8.8岁）的外周静脉血中的淋巴细胞亚群进行研究。研究了血清嗜酸性粒细胞阳离子蛋白（ECP）和髓过氧化物酶（MPO）浓度及其之间的关联、淋巴细胞亚群和肺功能。14名足月出生的健康儿童（中位年龄9.1岁）作为对照。使用流式细胞术测定T淋巴细胞（CD3）、T淋巴细胞亚群（CD4和CD8）、B淋巴细胞（CD19）、自然杀伤细胞（CD16+56）以及T和B淋巴细胞的活化标志物（CD23和CD25）。所有儿童均在诊所和家中测量肺功能（维达福数据存储肺活量计）。
结果
与对照组相比，极早产出生的学龄儿童的CD4(+)T细胞百分比和CD4:CD8比值显著更低（两者均p<0.05），而自然杀伤细胞百分比和血清ECP值显著更高（p<0.05）。极早产出生的学龄儿童的肺量计测量值显著低于对照组（p<0.05）——除用力肺活量外。当将所有受试者一起考虑时，在家中监测期间吸入β(2)激动剂后，呼气峰值流速时的支气管反应性与CD4(+)T细胞百分比（r = -0.45；p = 0.008）和CD4:CD8比值（r = -0.50；p = 0.003）之间观察到微弱但显著的负相关，表明支气管易激性与T细胞亚群失衡之间存在关联。
结论
这些结果表明，极早产出生的学龄儿童肺功能异常存在炎症基础。
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