Watson S J, Ellison J P
Histochemistry. 1976;50(2):119-27. doi: 10.1007/BF00495823.
This paper offers a technique for obtaining monoamine histofluorescence in the CNS by means of formaldehyde perfusion followed by cryostat sectioning. No freeze-drying is involved. Cryostat sections are exposed to formaldehyde vapor to complete the fluorophore formation. The fluorescence thus obtained is bright, well localized, and does not require loading the animals with precursors. The anatomical distribution of the pathways is identical to that obtained with the classical technique. Furthermore, the fluorescence is reversible by sodium borohydride, and exhibits the expected changes in intensity with pharmacological manipulations. The sections can be exposed to a cold aqueous medium for as long as 15 min with minimal diffusion of fluorophore; this suggests potential for combining monoamine histofluorescence with other visualization techniques.
本文提供了一种通过甲醛灌注继以低温恒冷箱切片在中枢神经系统中获得单胺组织荧光的技术。不涉及冷冻干燥。低温恒冷箱切片暴露于甲醛蒸汽中以完成荧光团的形成。由此获得的荧光明亮、定位良好，且无需给动物注射前体。该通路的解剖分布与经典技术所获得的相同。此外，荧光可被硼氢化钠逆转，并在药理学操作下呈现预期的强度变化。切片可暴露于冷的水性介质中长达15分钟，荧光团扩散极少；这表明单胺组织荧光与其他可视化技术相结合具有潜力。
