储存在添加剂溶液5中并在25摄氏度下暴露24小时的自体人红细胞的活力。

The viability of autologous human red cells stored in additive solution 5 and exposed to 25 degrees C for 24 hours.

作者信息

Reid T J, Babcock J G, Derse-Anthony C P, Hill H R, Lippert L E, Hess J R

机构信息

Blood Research Detachment, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA.

出版信息

Transfusion. 1999 Sep;39(9):991-7. doi: 10.1046/j.1537-2995.1999.39090991.x.

DOI:10.1046/j.1537-2995.1999.39090991.x

PMID:10533826

Abstract

BACKGROUND

No data exist on the viability of red cells (RBCs) stored in modern additive solution systems and allowed to warm above 10 degrees C.

STUDY DESIGN AND METHODS

In a randomized crossover study, 3 units of blood were collected at least 8 weeks apart from 11 volunteer donors and stored in additive solution 5 (AS-5). Of 3 units from each volunteer, 1 was stored for 6 weeks at 4 degrees C, 1 for 5 weeks at 4 degrees C except for 24 hours at 25 degrees C on Day 14, and 1 for 5 weeks at 4 degrees C except for 24 hours at 25 degrees C on Day 28. Units were sampled periodically during storage; at the end of storage, viability was measured by the 99mTc/51 Cr double-label method.

RESULTS

RBC viability was not significantly different in the storage protocols. Less than 1 percent of stored cells hemolyzed. RBC ATP concentrations at the end of storage correlated with viability and were approximately equal in the warmed units after 30 days' storage and the conventionally stored units after 42 days.

CONCLUSIONS

The data suggest that RBCs stored in AS-5 and allowed to warm to 25 degrees C for 24 hours lose about 12 days of their shelf life.

摘要

背景

目前尚无关于储存在现代添加剂溶液系统中且温度升至10摄氏度以上的红细胞（RBC）活力的数据。

研究设计与方法

在一项随机交叉研究中，从11名志愿献血者身上至少间隔8周采集3个单位的血液，并储存在添加剂溶液5（AS-5）中。每位志愿者的3个单位血液中，1个在4摄氏度下储存6周，1个在4摄氏度下储存5周，但在第14天有24小时处于25摄氏度，1个在4摄氏度下储存5周，但在第28天有24小时处于25摄氏度。储存期间定期对血液单位进行采样；储存结束时，通过99mTc/51Cr双标记法测量活力。